Dedicated Image Analysis Software Tool for the Evaluation of the Resorption Activity of Cultured Osteoclasts

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Abstract:

A semi-automated image analyzing system was developed for the quantitative study of the in vitro osteoclast activity. It is able to determine the percentage of the matrix reabsorbed by cultured osteoclasts. It counts and sizes the worn areas and displays the results together with two-color coding useful for visual interpretation. A specific semi-automated procedure was designed to simulate human sight. It detects local gray level differences while matching with the surrounding gray levels. It implements routine calculation enabling the operator to continuously control the process. We were able to accurately and precisely quantify the reabsorbed percentage of meaningful fields in a slice. This image-analyzing system is useful in the study of physiological or pathological osteoclast activity, in various diseases involving bone, such as osteoporosis, Paget's disease of bone or neoplastic conditions, and in the evaluation of osteoclast response to various agents.

Document Type: Research Article

DOI: http://dx.doi.org/10.2352/J.ImagingSci.Technol.(2008)52:3(030508)

Affiliations: 1: Department of Internal Medicine, University of Torino, Corso Dogliotti 14, 10126 Torino, Italy 2: Department of Mechanics, Politecnico of Torino, Corso Duca degli Abruzzi, 24, 10129 Torino, Italy 3: Department of Medical Oncology Center on Experimental Medicine (CeRMS), University of Torino, Via Santena 5 bis, 10126 Torino, Italy

Publication date: May 1, 2008

More about this publication?
  • The Journal of Imaging Science and Technology (JIST) is dedicated to the advancement of imaging science knowledge, the practical applications of such knowledge, and how imaging science relates to other fields of study. The pages of this journal are open to reports of new theoretical or experimental results, and to comprehensive reviews. Only original manuscripts that have not been previously published, nor currently submitted for publication elsewhere, should be submitted.

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