Evaluation of BrdU Influence on Sister Chromatid Exchange in Arctic and Silver Fox
The sister chromatid exchange (SCE) test is a cytogenetic assay and is known as a biomonitoring test to analyse chromosome damage caused by exogenous factors. The aim of the study was to analyse chromatin instability by determining the number and sites of spontaneous SCE in the karyotype of arctic and silver foxes. Twenty-four animals: 12 arctic foxes (Vulpes lagopus) and 12 silver foxes (Vulpes vulpes) were investigated. The experiment used peripheral blood lymphocytes following in vitro culture. Karyotype evaluation in both fox species was made by RBA staining with modified Giemsa reagent. To analyse SCE, three different bromodeoxyuridine (BrdU) concentrations were added to the culture and the microscopic preparations were stained by the FPG technique. Based on these results, chromosomal polymorphism in the arctic fox and the modal number of B chromosomes in the silver fox were established. Spontaneous SCE in both fox species was observed at a concentration of 0.5 μg/ml BrdU. The number of spontaneous SCE averaged 0.65±0.55 SCE/cell in the arctic fox and 2.33±0.76 SCE/cell in the silver fox. Higher BrdU concentrations induced additional chromatin breaks. The arctic fox was characterized by a greater number of centromeric SCE in relation to the other SCE types. In the silver fox, centromeric and terminal SCE occurred at a similar frequency. No significant effect of the living environment and no effect of sex on increasing SCE frequency were found in either fox species.
No Supplementary Data.
No Article Media
Document Type: Research Article
Publication date: 2017-09-01
More about this publication?
- Folia biologica is an international quarterly journal that publishes papers on the broad field of experimental zoology, nuclear and chromosome research, and also ultrastructural studies. All papers are subject to peer reviews. Indexed in: ISI Master Journal List, Current Contents, Polish Scientific Journals Contents. I.F. 0.667
- Editorial Board
- Information for Authors
- Ingenta Connect is not responsible for the content or availability of external websites