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Free Content Use of Cytochrome b Polymorphism for Species Identification of Biological Material Derived from Cattle, Sheep, Goats, Roe Deer and Red Deer

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The objective of this study was species identification of the following biological trace material: skin, blood stains, meat samples and jawbone with a tooth, which were the subject of expert opinion ordered by a court. The expert appraisement was conducted by an analysis of a cytochrome b fragment. The choice of mtDNA fragment for analysis was based on its conservation in mammals which enabled several farm and wild species to be identified with one pair of primers. The PCR product was differentiated by Tsp509I and AluI enzymes. Due to problems with amplification of roe deer DNA, primers specific to this species only, flanking a cytochrome b fragment (Y14951.1), were designed. On the basis of this analysis, it was concluded that the skin sample was derived from a goat, dried blood from a roe deer, the jawbone from cattle, and two meat samples from a roe deer and red deer. This method allowed rapid and efficient identification of several species of mammals using diverse biological material.

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Keywords: CYTOCHROME B; EXPERT OPINION; PCR-RFLP; SPECIES IDENTIFICATION

Document Type: Research Article

Publication date: 01 December 2009

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  • Folia biologica is an international quarterly journal that publishes papers on the broad field of experimental zoology, nuclear and chromosome research, and also ultrastructural studies. All papers are subject to peer reviews. Indexed in: ISI Master Journal List, Current Contents, Polish Scientific Journals Contents. I.F. 0.667
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