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Open Access Retrieval of hundreds of nuclear loci from herbarium specimens

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Herbaria are unparalleled collections of biodiversity information representing the world's flora. However, this treasure has remained largely inaccessible to genetic studies, frequently limited by the low yields of poor-quality DNA. Next-generation sequencing (NGS) has transformed every field of biological research. The different strategies for accessing genetic data using NGS are changing the direction of biodiversity research—we are no longer constrained by a relatively small number of markers for non-model organisms, by time and cost limited sample sizes, or by incomplete datasets due to recalcitrant DNA extractions or PCR amplification failure. Here we show that targeted enrichment through hybrid capture can be used to generate hundreds of kilobases of nuclear sequence data of the Neotropical genus Inga, from herbarium specimens as old as 180 years and using as little as 16 ng of degraded DNA.
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Keywords: DEGRADED DNA; HERBARIUM SPECIMEN; HYBRID BAITS; HYBRID CAPTURE; INGA

Document Type: Research Article

Affiliations: 1: Royal Botanic Garden Edinburgh, 20A Inverleith Row, Edinburgh EH3 5LR, U.K. 2: Royal Botanic Garden Edinburgh, 20A Inverleith Row, Edinburgh EH3 5LR, U.K., Institute of Evolutionary Biology, University of Edinburgh, Edinburgh EH9 3FL, U.K. 3: Royal Botanic Garden Edinburgh, 20A Inverleith Row, Edinburgh EH3 5LR, U.K., Institute of Molecular Plant Sciences, University of Edinburgh, Edinburgh EH9 3BF, U.K.;, Email: [email protected]

Publication date: 01 October 2016

This article was made available online on 12 October 2016 as a Fast Track article with title: "Retrieval of hundreds of nuclear loci from herbarium specimens".

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