Skip to main content

A rapid and cost-efficient DMSO-based method for isolating DNA from cultured lichen photobionts

Buy Article:

$15.60 plus tax (Refund Policy)

We have developed a simple and fast procedure for the purification of PCR-quality DNA from cultured lichen photobionts. This new one-step method uses the solvent dimethyl sulfoxide (DMSO) combined with heat treatment to chemically breakdown algal and plant tissues. The DMSO-DNA extracts may be directly precipitated and purified by standard techniques in a total time of approximately 30 min. Compared to other DNA extraction protocols, the DMSO-based method suppresses the need for liquid nitrogen, any extraction buffer, grinding, phenol, and long incubation or centrifugation steps, thereby considerably reducing the possibility of contamination. In addition, minimal amounts of starting material (5 × 106–20 × 106 cells from liquid or agarized cultures) produce sufficient DNA for 200 PCR reactions approximately, making this protocol a practical option for colony screening. This method works well in a wide range of cultured lichen photobionts and reduces the amount of labor-intensive steps and time consumed by other multi-step procedures, allowing for efficient processing of an increased number of samples.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics

Keywords: ALGAE; CYANOBACTERIA; DMSO; DNA ISOLATION; LICHENS; PHOTOBIONT; PLANTS

Document Type: Research Article

Affiliations: 1: Department of Plant Biology, University of Alcalá, 28871-Alcalá de Henares (Madrid), Spain;, Email: [email protected] 2: Department of Plant Biology, University of Alcalá, 28871-Alcalá de Henares (Madrid), Spain 3: ICBIBE, Department of Botany, University of Valencia, Faculty of Biology, C/ Dr. Moliner 50, 46100-Burjassot (Valencia), Spain

Publication date: 2010-04-01

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more