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A proposal for a standardised protocol to barcode all land plants

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Abstract:

We propose in this paper to use three regions of plastid DNA as a standard protocol for barcoding all land plants. We review the other markers that have been proposed and discuss their advantages and disadvantages. The low levels of variation in plastid DNA make three regions necessary; there are no plastid regions, coding or non-coding, that evolve as rapidly as mitochondrial DNA generally does in animals. We outline two, three-region options, (1) rpoC1, rpoB and matK or (2) rpoC1, matK and psbA-trnH as viable markers for land plant barcoding.

Keywords: ITS NRDNA; LAND PLANT BARCODING; MATK; MITOCHONDRIAL DNA; PLASTID DNA; PSBA-TRNH; RBCL; RPOB; RPOC1; TRNL

Document Type: Short Communication

Affiliations: 1: Jodrell Laboratory, Royal Botanic Gardens, Kew, Richmond, Surrey, TW9 3DS, U.K. 2: Royal Botanic Garden, Inverleith Row, Edinburgh, EH3 5LR, U.K. 3: Departamento de Ciências Biológicas-DCBIO, Universidade Estadual de Feira de Santana-UEFS, BR116 Km3 Campus Universitário, Feira de Santana – BA, CEP 44031-460, Brazil 4: Departamento de Ciencias Biológicas, Universidad de los Andes, Apartado Aéreo 4976, Bogotá, D.C., Colombia 5: The Natural History Museum of Denmark, Sølvgade 83, Opg. S, 1307 Copenhagen K, Denmark 6: The Lewis B. and Dorothy Cullman Program for Molecular Systematics Studies, The New York Botanical Garden, Bronx, New York 10458-5126, U.S.A. 7: Department of Botany, The Natural History Museum, Cromwell Road, London SW7 5BD, U.K. 8: Bolus Herbarium, Department of Botany, University of Cape Town, 7701 Rondebosch, South Africa 9: Leslie Hill Molecular Systematics Laboratory, Kirstenbosch Research Centre, Private Bag X7, Claremont 7735, Cape Town, South Africa 10: Departamento de Botánica, Instituto de Biología, Universidad Nacional Autonóma de México, Apartado Postal 70-367, 04510 México, D.F., México 11: Imperial College London and Royal Botanic Gardens, Kew, Division of Biology, Silwood Park Campus, Ascot, Berkshire SL5 7PY, U.K. 12: Institute of Biological Sciences, Edward Llwyd Building, The University of Wales, Aberystwyth, Ceredigion, SY23 3DA, U.K.

Publication date: May 1, 2007

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