If you are experiencing problems downloading PDF or HTML fulltext, our helpdesk recommend clearing your browser cache and trying again. If you need help in clearing your cache, please click here . Still need help? Email help@ingentaconnect.com

Using GenBank data for plant identification: possibilities and limitations using the ITS 1 of Asteraceae species belonging to the tribes Lactuceae and Anthemideae

$17.95 plus tax (Refund Policy)

Buy Article:

Abstract:

As a contribution to establish taxonomic identification methods using DNA sequence data, we evaluate to which extent the present set-up of the nucleotide databases (NCBI, EBI, GenomeNet) allows to use them for reliable routine plant identification applying the implemented sequence similarity and homology search tools. We screened newly determined ITS 1 sequences from Asteraceae (tribes Lactuceae, Anthemideae) against the molecular nucleotide databases where sequences from that exact species, from other species of the same genus, or only from other genera of the same family had already been published. With the present set up of the BLAST® algorithms [MEGABLAST, BLASTN] 100% correct family, 99% correct tribe, and 98% precise genus affiliation could be achieved in Lactuceae representatives and 100% correct family, 100% correct tribe, and 95% precise genus affiliation in Anthemideae. Correct species detection varied between 86% accuracy for Lactuceae and 91% in Anthemideae. Necessary optimization criteria are proposed as well as further requirements for a standardized molecular identification system.

Keywords: ASTERACEAE; DNA TAXONOMY; ITS; MOLECULAR MARKER; PLANT IDENTIFICATION

Document Type: Research Article

Affiliations: 1: Botanic Garden and Botanical Museum Berlin-Dahlem (FU-Berlin), Königin-Luise-Straße 6–8, D-14191 Berlin, Germany 2: Institute of Botany, University of Regensburg, Universitätsstr. 31, D-93053 Regensburg, Germany 3: Institute for Plant Genetics and Crop Plant Research (IPK), Corrensstraße 3, D-06466 Gatersleben, Germany

Publication date: February 1, 2006

Related content

Tools

Favourites

Share Content

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more