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Successful extraction of DNA from 100-year-old herbarium specimens of the liverwort Bazzania trilobata

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Using a modified DNeasy™ Plant Mini Kit method to extract DNA, we were able to isolate DNA from 100year-old liverwort herbarium specimens. We sucessfully isolated DNA from 18 specimens of the liverwort Bazzania trilobata collected during the 20th century. Our observations suggest that, at least over 100 years of material storage, the most crucial factor in successful DNA isolation is method of drying, not age of sample.
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Keywords: BAZZANIA TRILOBATA; DNA ISOLATION; LIVERWORT; METHOD OF DRYING; PCR

Document Type: Research Article

Affiliations: 1: Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, Miedzychodzka 5, 60-371 Poznan, Poland 2: Department of Genetics, Institute of Experimental Biology, Adam Mickiewicz University, Miedzychodzka 5, 60-371 Poznan, Poland

Publication date: 2005-05-01

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