PCR amplification of Michele Tenore's historical specimens and facility to utilize an alternative approach to resolve taxonomic problems
Abstract:A polymorphic non-coding region of chloroplast DNA (cpDNA) was amplified by the polymerase chain reaction (PCR) from herbarium specimens of Pinus brutia and P. halepensis. The samples belong to the historical collection of Michele Tenore. Several discordances and inconsistencies in taxonomic attribution are present for historical specimens of P. brutia. Moreover, there are inaccuracies as to their geographical distribution. In this work, we demonstrate the potential use of molecular methods of amplifying DNA from historical samples to resolve the identification of herbarium specimens.
Document Type: Research Article
Affiliations: 1: Dipartimento di Biologia Vegetale, Università degli Studi di Napoli "Federico II", Via Foria 223, Napoli I80139, Italy. 2: Orto Botanico, Università degli Studi di Napoli "Federico II", Via Foria 223, Napoli I-80139, Italy.
Publication date: February 1, 2004
Impact Factor (2014): 3.3
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