Skip to main content

Evaluation of Seven Different Commercially Available Real-Time PCR Assays for Detection of Shiga Toxin 1 and 2 Gene Subtypes

Buy Article:

$37.00 plus tax (Refund Policy)

Abstract:

Following the recent outbreak of Shiga toxin–producing Escherichia coli (STEC) O104:H4 infection in Germany, the demand for fast detection of STEC has again increased. Various real-time PCR-based methods enabling detection of Shiga toxin genes (stx) have been developed and can be used for applications in food microbiology. The present study was conducted to evaluate the reliability of seven commercially available real-time PCR systems for detection of stx 1 and stx 2 subtypes. For this purpose, pure cultures of 18 STEC strains harboring all known stx 1 and/or stx 2 subtypes were tested. Only one of the seven real-time PCR systems detected all known stx 1 and stx 2 subtypes. Six systems failed to detect the stx 2f subtype. One system missed stx 2 subtypes reported in association with severe human disease. Because the presence of certain stx genes (subtypes) is considered an important indicator of STEC virulence, systems differentiating between the stx 1 and stx 2 gene groups provide added value. Reliable and fast detection of stx genes is of major importance for both diagnostic laboratories and the food industry.

Document Type: Research Article

DOI: http://dx.doi.org/10.4315/0362-028X.JFP-12-365

Affiliations: 1: Institute for Food Safety and Hygiene, Vetsuisse Faculty University of Zurich, CH-8057 Zurich, Switzerland 2: Department of Quality and Safety, Nestlé Research Centre, Vers-chez-les-Blanc, CH-1000 Lausanne, Switzerland 3: Institute for Food Safety and Hygiene, Vetsuisse Faculty University of Zurich, CH-8057 Zurich, Switzerland. stephanr@fsafety.uzh.ch

Publication date: May 1, 2013

More about this publication?
  • IAFP Members with personal subscriptions to JFP Online: To access full-text JFP or JMFT articles, you must sign-in in the upper-right corner using your Ingenta sign-in details (your IAFP Member Login does not apply to this website).

    The Journal of Food Protection (JFP) is a refereed monthly publication. Each issue contains scientific research and authoritative review articles reporting on a variety of topics in food science pertaining to food safety and quality. The Journal is internationally recognized as the leading publication in the field of food microbiology with a readership exceeding 11,000 scientists from 70 countries. The Journal of Food Protection is indexed in Index Medicus, Current Contents, BIOSIS, PubMed, Medline, and many others.

    Print and online subscriptions are available to IAFP Members and institutional subscribers. IAFP Members with a subscription to JFP Online will have access to all available JFP and JMFT content. Online visitors who are not IAFP Members or journal subscribers will be charged on a pay-per-view basis. Membership and subscription information is available at www.foodprotection.org.
  • Information for Authors
  • Submit a Paper
  • Subscribe to this Title
  • Membership Information
  • Information for Advertisers
  • ingentaconnect is not responsible for the content or availability of external websites
iafp/jfp/2013/00000076/00000005/art00018
dcterms_title,dcterms_description,pub_keyword
6
5
20
40
5

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more