Influence of Glucose Concentrations on Biofilm Formation, Motility, Exoprotease Production, and Quorum Sensing in Aeromonas hydrophila
Source: Journal of Food Protection®, Number 2, February 2013, pp. 190-369 , pp. 239-247(9)
Abstract:Aeromonas hydrophila recently has received increased attention because it is opportunistic and a primary human pathogen. A. hydrophila biofilm formation and its control are a major concern for food safety because biofilms are related to virulence. Therefore, we investigated biofilm formation, motility inhibition, quorum sensing, and exoprotease production of this opportunistic pathogen in response to various glucose concentrations from 0.05 to 2.5% (wt/vol). More than 0.05% glucose significantly impaired (P < 0.05) quorum sensing, biofilm formation, protease production, and swarming and swimming motility, whereas bacteria treated with 0.05% glucose had activity similar to that of the control (0% glucose). A stage shift biofilm assay revealed that the addition of glucose (2.5%) inhibited initial biofilm formation but not later stages. However, addition of quorum sensing molecules N-3-butanoyl-DL-homoserine lactone and N-3-hexanoyl homoserine lactone partially restored protease production, indicating that quorum sensing is controlled by glucose concentrations. Thus, glucose present in food or added as a preservative could regulate acyl-homoserine lactone quorum sensing molecules, which mediate biofilm formation and virulence in A. hydrophila.
Document Type: Research Article
Affiliations: 1: School of Food Science and Technology, Chung-Ang University, 72–1 Nae-Ri, Daedeok-Myun, Ansung, Kyunggido 456–756, South Korea, Department of Microbiology, Jessore Science and Technology University, Jessore 7408, Bangladesh 2: School of Food Science and Technology, Chung-Ang University, 72–1 Nae-Ri, Daedeok-Myun, Ansung, Kyunggido 456–756, South Korea 3: School of Food Science and Technology, Chung-Ang University, 72–1 Nae-Ri, Daedeok-Myun, Ansung, Kyunggido 456–756, South Korea;, Email: firstname.lastname@example.org
Publication date: February 1, 2013
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