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Influence of Glucose Concentrations on Biofilm Formation, Motility, Exoprotease Production, and Quorum Sensing in Aeromonas hydrophila

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Aeromonas hydrophila recently has received increased attention because it is opportunistic and a primary human pathogen. A. hydrophila biofilm formation and its control are a major concern for food safety because biofilms are related to virulence. Therefore, we investigated biofilm formation, motility inhibition, quorum sensing, and exoprotease production of this opportunistic pathogen in response to various glucose concentrations from 0.05 to 2.5% (wt/vol). More than 0.05% glucose significantly impaired (P < 0.05) quorum sensing, biofilm formation, protease production, and swarming and swimming motility, whereas bacteria treated with 0.05% glucose had activity similar to that of the control (0% glucose). A stage shift biofilm assay revealed that the addition of glucose (2.5%) inhibited initial biofilm formation but not later stages. However, addition of quorum sensing molecules N-3-butanoyl-DL-homoserine lactone and N-3-hexanoyl homoserine lactone partially restored protease production, indicating that quorum sensing is controlled by glucose concentrations. Thus, glucose present in food or added as a preservative could regulate acyl-homoserine lactone quorum sensing molecules, which mediate biofilm formation and virulence in A. hydrophila.

Document Type: Research Article

DOI: http://dx.doi.org/10.4315/0362-028X.JFP-12-321

Affiliations: 1: School of Food Science and Technology, Chung-Ang University, 72–1 Nae-Ri, Daedeok-Myun, Ansung, Kyunggido 456–756, South Korea, Department of Microbiology, Jessore Science and Technology University, Jessore 7408, Bangladesh 2: School of Food Science and Technology, Chung-Ang University, 72–1 Nae-Ri, Daedeok-Myun, Ansung, Kyunggido 456–756, South Korea 3: School of Food Science and Technology, Chung-Ang University, 72–1 Nae-Ri, Daedeok-Myun, Ansung, Kyunggido 456–756, South Korea;, Email: sangdoha@cau.ac.kr

Publication date: February 1, 2013

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    First published in 1937, the Journal of Food Protection®, is a refereed monthly publication. Each issue contains scientific research and authoritative review articles reporting on a variety of topics in food science pertaining to food safety and quality. The Journal is internationally recognized as the leading publication in the field of food microbiology with a readership exceeding 11,000 scientists from 70 countries. The Journal of Food Protection® is indexed in Index Medicus, Current Contents, BIOSIS, PubMed, Medline, and many others.

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