Antibiotic-Resistant Enterococcus faecalis in Abattoir Pigs and Plasmid Colocalization and Cotransfer of tet(M) and erm(B) Genes

Authors: Tremblay, Cindy-Love1; Letellier, Ann1; Quessy, Sylvain1; Daignault, Danielle2; Archambault, Marie3

Source: Journal of Food Protection®, Number 9, September 2012, pp. 1548-1720 , pp. 1595-1602(8)

Publisher: International Association for Food Protection

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Abstract:

This study was conducted to determine plasmid colocalization and transferability of both erm(B) and tet(M) genes in Enterococcus faecalis isolates from abattoir pigs in Canada. A total of 124 E. faecalis isolates from cecal contents of abattoir pigs were examined for antibiotic susceptibility. High percentages of resistance to macrolides and tetracyclines were found. Two predominant multiresistance patterns of E. faecalis were examined by PCR and sequencing for the presence of genes encoding antibiotic resistance. Various combinations of antibiotic resistance genes were detected; erm(B) and tet(M) were the most common genes. Plasmid profiling and hybridization revealed that both genes were colocated on a ∼9-kb transferable plasmid in six strains with the two predominant multiresistant patterns. Plasmid colocalization and cotransfer of tet(M) and erm(B) genes in porcine E. faecalis isolates indicates that antibiotic coselection and transferability could occur via this single genetic element. To our knowledge, this is the first report on plasmid colocalization and transferability of erm(B) and tet(M) genes in E. faecalis on a mobile genetic element of ∼9 kb. Physical linkage between important antibiotic resistance determinants in enterococci is of interest for predicting potential transfer to other bacterial genera.

Document Type: Research Article

DOI: http://dx.doi.org/10.4315/0362-028X.JFP-12-047

Affiliations: 1: Swine Infectious Disease Research Center, Department of Pathology and Microbiology, University of Montreal, Faculty of Veterinary Medicine, 3200 Sicotte Street, Saint-Hyacinthe, Quebec, Canada J2S 7C6 2: Public Health Agency of Canada, Laboratory for Foodborne Zoonoses, 3400 Casavant Boulevard W., Saint-Hyacinthe, Quebec, Canada J2S 8E3 3: Swine Infectious Disease Research Center, Department of Pathology and Microbiology, University of Montreal, Faculty of Veterinary Medicine, 3200 Sicotte Street, Saint-Hyacinthe, Quebec, Canada J2S 7C6. marie.archambault@umontreal.ca

Publication date: September 1, 2012

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    First published in 1937, the Journal of Food Protection®, is a refereed monthly publication. Each issue contains scientific research and authoritative review articles reporting on a variety of topics in food science pertaining to food safety and quality. The Journal is internationally recognized as the leading publication in the field of food microbiology with a readership exceeding 11,000 scientists from 70 countries. The Journal of Food Protection® is indexed in Index Medicus, Current Contents, BIOSIS, PubMed, Medline, and many others.

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