Colloidal Gold–Based Immunochromatographic Test Strip for Rapid Detection of Abrin in Food Samples
Abstract:In the present study, we developed a convenient, rapid, and sensitive immunochromatographic (IC) test strip to detect abrin in assay buffer and spiked abrin in test food samples. The abrin IC test strip was based on a sandwich format consisting of a monoclonal antibody and a polyclonal antibody. The anti-abrin A chain monoclonal antibody from mice was immobilized on a porous nitrocellulose membrane as a capture antibody, while the anti-abrin polyclonal antibody from rabbits was conjugated to colloidal gold particles, serving as a detection antibody. Both visual observation and quantitative analysis indicated that the lower detection of the strip was about 3 ng/ml when abrin was directly spiked into milk, orange juice, and drinking water at a concentration of 3 to 60 ng/ml; the analytical recovery rate was 92.2 to 128%. With this method, abrin spiked into food could be detected in less than 10 min. Moreover, the IC test strip showed no cross-reaction with the closely related phytotoxin ricin. Therefore, our test strip is an ideal candidate for the development of a kit for rapid and quantitative detection of abrin in food samples.
Document Type: Research Article
Affiliations: 1: State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, No. 20 Dongda Street, Fengtai District, Beijing 100071, People's Republic of China 2: Chinese Academy of Inspection and Quarantine, Institute of Health Quarantine, No. A3, Gaobeidian Bei Lu, Chaoyang District, Beijing 100123, People's Republic of China 3: State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, No. 20 Dongda Street, Fengtai District, Beijing 100071, People's Republic of China. firstname.lastname@example.org
Publication date: January 1, 2012
- IAFP Members with personal subscriptions to JFP Online: To access full-text JFP or JMFT articles, you must sign-in in the upper-right corner using your Ingenta sign-in details (your IAFP Member Login does not apply to this website). The Journal of Food Protection (JFP) is a refereed monthly publication. Each issue contains scientific research and authoritative review articles reporting on a variety of topics in food science pertaining to food safety and quality. The Journal is internationally recognized as the leading publication in the field of food microbiology with a readership exceeding 11,000 scientists from 70 countries. The Journal of Food Protection is indexed in Index Medicus, Current Contents, BIOSIS, PubMed, Medline, and many others.
Print and online subscriptions are available to IAFP Members and institutional subscribers. IAFP Members with a subscription to JFP Online will have access to all available JFP and JMFT content. Online visitors who are not IAFP Members or journal subscribers will be charged on a pay-per-view basis. Membership and subscription information is available at www.foodprotection.org.
- Information for Authors
- Submit a Paper
- Subscribe to this Title
- Membership Information
- Information for Advertisers
- ingentaconnect is not responsible for the content or availability of external websites