Inactivation of Feline Calicivirus as a Surrogate for Norovirus on Lettuce by Electron Beam Irradiation
Abstract:Caliciviridae, including norovirus, are considered important sources of human gastroenteritis. As leafy green vegetables are commonly consumed without additional processing, it is important to evaluate interventions to reduce the presence of human pathogens in these products. Feline calicivirus was used as a model for small round structured viruses on lettuce. The lettuce was inoculated by immersion to simulate contamination from irrigation or wash water. The inoculated lettuce was then exposed to electron beam irradiation at various dose levels to determine survival. The D 10-value of the calicivirus on lettuce was determined to be 2.95 kGy. Irradiation to reduce bacterial pathogens on cut lettuce could also reduce the risk associated with small round structured viruses on lettuce.
Document Type: Research Article
Affiliations: 1: Department of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, Iowa 50011, USA 2: Department of Animal Science, Iowa State University, Ames, Iowa 50011, USA 3: Department of Animal Science, Iowa State University, Ames, Iowa 50011, USA; Inter-Departmental Program in Microbiology, Iowa State University, Ames, Iowa 50011, USA
Publication date: September 1, 2011
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