Molecular Epidemiology and Antimicrobial Susceptibility of Campylobacter jejuni and Campylobacter coli Isolates of Poultry, Swine, and Cattle Origin Collected from Slaughterhouses in Hungary

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Abstract:

Campylobacter spp. are the most common cause of bacterial enteritis in Hungary, and the aim of this study was to identify the distribution, genotypes, and antimicrobial susceptibility of Campylobacter species in the most important food-producing animals at the time of slaughter during 2008 and 2009. Of 1,110 samples, 266 were identified as Campylobacter coli (23.9%) and 143 as C. jejuni (12.9%) by real-time PCR. Resistance to enrofloxacin-ciprofloxacin and nalidixic acid was significant, especially in C. jejuni (73.3%) and C. coli (77.2%) from broilers. Higher erythromycin (P = 0.043) and tetracycline (P = 1.865e – 14) resistance rates were found among C. coli isolates (9.7 and 74.1%, respectively) than among C. jejuni isolates (3.1 and 36.6%, respectively). A total of 47 fla short variable region sequences were identified among 73 selected C. coli and C. jejuni isolates, with 35 fla types detected only once. At the nucleotide level, fla types A66 and A21 were the most common. Using the pulsed-field gel electrophoresis method, 66% of strains exhibited unique profiles after Sma I digestion. Forty-two isolates assigned to 18 Sma I clusters were further typed by Kpn I, and of these, 24 were assigned to 10 Kpn I clusters. For isolates in five Kpn I clusters, epidemiological links were observed. Stable C. jejuni and C. coli clones were detected, indicating that further studies involving broiler and human isolates need to be conducted to elucidate the importance of these stable clones in human infections.

Document Type: Research Article

DOI: http://dx.doi.org/10.4315/0362-028X.JFP-10-376

Affiliations: 1: Department of Bacteriology, Central Agricultural Office Veterinary Diagnostic Directorate, Tábornok u. 2., 1149 Budapest, Hungary. schweitzer.nora@gmail.com 2: Department of Molecular Biology, Central Agricultural Office Veterinary Diagnostic Directorate, Tábornok u. 2., 1149 Budapest, Hungary 3: Department of Bacteriology, Central Agricultural Office Veterinary Diagnostic Directorate, Tábornok u. 2., 1149 Budapest, Hungary 4: Department of Microbiology and Infectious Diseases, Faculty of Veterinary Science, Szent István University, Hungária krt. 23-25., 1143 Budapest, Hungary 5: National Centre for Epidemiology, Phage and Molecular Typing Department, Gyáli út 2-6., 1097 Budapest, Hungary

Publication date: June 1, 2011

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