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Incidence of Fumonisin B2 Production within Aspergillus Section Nigri Populations Isolated from California Raisins

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Abstract:

Fungi belonging to Aspergillus section Nigri occur frequently and in high populations on grapes. Species within this section include Aspergillus niger, A. tubingensis, and A. carbonarius, and they are potential sources for mycotoxins including ochratoxin A and fumonisin B2 (FB2) in grapes and grape products. Aspergillus section Nigri strains were isolated from California raisins to examine the frequency and extent of FB2 production. Of 392 strains isolated, 197 strains were identified as A. niger, 131 of which produced FB2. These strains produced from 1.2 to 27 μg/ml FB2 in culture. PCR amplification of fum1 and fum19 gene fragments showed that all FB2-producing strains and nearly all nonproducing strains of A. niger contain these genes. An additional 175 strains were identified as A. tubingensis, none of which produced FB2. PCR with fum1 and fum19 primers amplified gene fragments of 14 and 25% of A. tubingensis strains, respectively, suggesting that putative orthologs of A. niger fumonisin biosynthetic genes might occur in A. tubingensis. These results indicate that FB2 production is common among field isolates of A. niger and suggest that the potential for FB2 contamination of California raisins should be addressed further.

Document Type: Research Article

DOI: http://dx.doi.org/10.4315/0362-028X.JFP-10-412

Affiliations: 1: Plant Mycotoxin Research Unit, U.S. Department of Agriculture, Agricultural Research Service, 800 Buchanan Street, Albany, California 94710, USA. jeffrey.palumbo@ars.usda.gov 2: Plant Mycotoxin Research Unit, U.S. Department of Agriculture, Agricultural Research Service, 800 Buchanan Street, Albany, California 94710, USA

Publication date: April 1, 2011

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    First published in 1937, the Journal of Food Protection®, is a refereed monthly publication. Each issue contains scientific research and authoritative review articles reporting on a variety of topics in food science pertaining to food safety and quality. The Journal is internationally recognized as the leading publication in the field of food microbiology with a readership exceeding 11,000 scientists from 70 countries. The Journal of Food Protection® is indexed in Index Medicus, Current Contents, BIOSIS, PubMed, Medline, and many others.

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