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Antimicrobial Resistance and Molecular Subtyping of Campylobacter jejuni and Campylobacter coli from Retail Meats

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Abstract:

Campylobacter isolates (n = 297; 202 C. jejuni and 95 C. coli isolates) recovered from 2,513 retail meat samples (chicken breasts, ground turkey, ground beef, and pork chops) were examined for antimicrobial susceptibility. The isolates were further analyzed for genetic relatedness by pulsed-field gel electrophoresis (PFGE) using SmaI and KpnI restriction enzymes, and a subset of isolates (n = 174) were subtyped by multilocus sequence typing (MLST). The resistance most frequently observed was that to doxycycline (27.6%), followed by ciprofloxacin (13.8%) and erythromycin (6.4%). All isolates were susceptible to gentamicin and meropenem. C. coli showed higher resistance to doxycycline than did C. jejuni (42.1 versus 20.8%) and lower resistance to ciprofloxacin than did C. jejuni (10.5 versus 15.3%). Erythromycin resistance was only observed in C. coli. PFGE using SmaI plus KpnI digestion generated 168 clusters from 297 isolates: 115 from C. jejuni and 53 from C. coli. MLST revealed 44 sequence types (STs) under 10 clonal complexes from 120 C. jejuni and 27 STs under two clonal complexes from 54 C. coli. There was a positive association between PFGE and STs; however, PFGE showed greater discriminatory power than MLST. Subtyping data did not correlate with antimicrobial resistance phenotypes.

Document Type: Research Article

DOI: http://dx.doi.org/10.4315/0362-028X.JFP-10-432

Affiliations: 1: College of Food Science and Engineering, Northwest A&F University, Yanglin, Shaanxi, China 2: Division of Animal and Food Microbiology, Office of Center for Veterinary Medicine, U.S. Food and Drug Administration, Laurel, Maryland, USA 3: College of Food Science and Engineering, Northwest A&F University, Yanglin, Shaanxi, China; Department of Nutrition and Food Science and Joint Institute for Food Science and Applied Nutrition (JIFSAN), University of Maryland, College Park, Maryland, USA. jmeng@umd.edu

Publication date: April 1, 2011

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