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Antibiotic Resistance and Virulence Factors among Enterococci Isolated from Chouriço, a Traditional Portuguese Dry Fermented Sausage

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Abstract:

Enterococci are ubiquitous microorganisms, found as part of the normal intestinal microbiota of many animals. They can be present in food products, for example, the Portuguese dry fermented sausage chouriço. Twenty enterococci were isolated from chouriço in two processing units; after identification and typification by conventional-molecular methods, the isolates were screened for virulence factors and antibiotic resistance. Identification allocated all enterococci to the species Enterococcus faecalis, and PCR fingerprinting demonstrated that each isolate was specific to the processing unit and chouriço from which it was recovered. Regarding the screening for virulence factors, 1 strain produced cytolysin and 4 were gelatinase positive, but none produced lipase. The ace gene was detected in 1 enterococci, ebpABC and efaAfs in 16 isolates each, esp in 3, fsrB in 5, gelE in 7, and cylA in 1. A multiresistant phenotype was observed in 8 isolates, 6 belonging to factory A. The antibiotic resistance gene ere(B) was detected in 9 enterococci, whereas the genes tet(M), aac(6′)-Ie-aph(2′′), and vanA were detected in 8 isolates each. As some of the E. faecalis chouriço isolates present a multiresistant profile and harbor virulence and/or resistance genes, to assess further the safety of Portuguese dry sausages, a larger number of products and processing units must by analyzed.

Document Type: Research Article

DOI: https://doi.org/10.4315/0362-028X.JFP-10-309

Affiliations: 1: Centro de Investigação Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterinária, Universidade Técnica de Lisboa, Avenida da Universidade Técnica, 1300-477 Lisbon, Portugal 2: Institute of Animal Physiology Slovak Academy of Sciences, Šoltésovej 4-6, 04001 Košice, Slovakia 3: Universidade de Évora, Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Apartado 94, 7002-554 Évora, Portugal 4: Universidade de Lisboa, Faculdade de Ciê;ncias, Centro de Biodiversidade, Genómica Integrativa e Funcional, Edifício ICAT, Campus da FCUL, Campo Grande, Lisbon, Portugal 5: Centro de Investigação Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterinária, Universidade Técnica de Lisboa, Avenida da Universidade Técnica, 1300-477 Lisbon, Portugal. tmsemedo@gmail.com tlemsaddek@fmv.utl.pt

Publication date: 2011-03-01

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