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The aim of this study was to investigate the potential of using Al-Holy–Rasco (AR) medium, a novel broth for detection and isolation of Cronobacter spp. in infant formula milk (IFM). The new medium's composition is generic brain heart infusion broth with the addition of
1% NaCl, 15% sucrose, and 0.80 g/liter sodium deoxycholate as selective ingredients. AR broth outperformed Enterobacteriaceae enrichment broth (EE), Enterobacter sakazakii enrichment broth (ESE), modified lauryl sulfate broth, and milk as enrichment media to stimulate the growth
of a cocktail of 10 strains of Cronobacter. Additionally, AR broth significantly suppressed the growth of competing non-Cronobacter Enterobacteriaceae as compared with EE, ESE, modified lauryl sulfate broth, and milk. The recovery of desiccated Cronobacter (1 to 5,000
CFU/100 g) from powdered IFM in the presence of competing non-Cronobacter Enterobacteriaceae was determined by EE, ESE, and AR broth with 10 and 15% sucrose. AR broth with 15% sucrose outperformed all other examined broths and recovered Cronobacter from all samples tested at
all Cronobacter concentrations. AR broth must be validated before it can be used for rapid detection and isolation of Cronobacter from powdered IFM.
Department of Clinical Nutrition and Dietetics, Faculty of Allied Health Sciences, Hashemite University, P.O. Box 150459, Zarqa-Jordan. firstname.lastname@example.org 2:
School of Food Science and Human Nutrition, Box 646376, Washington State University, Pullman, Washington 99164, USA 3:
Department of Nutrition and Food Technology, Faculty of Agriculture, Jordan University of Science and Technology, P.O. Box 3030, Irbid 22110, Jordan
Publication date: March 1, 2011
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