Role of Cellulose in Protecting Shiga Toxin–Producing Escherichia coli against Osmotic and Chlorine Stress
Authors: Yoo, Byong K.; Chen, Jinru
Source: Journal of Food Protection®, Number 11, November 2010, pp. 1962-2140 , pp. 2084-2088(5)
Abstract:This study was undertaken to determine the role of cellulose in protecting Shiga toxin–producing Escherichia coli (STEC) against osmotic and chlorine treatments. STEC cells producing cellulose (19B and 49B) and their respective cellulose-deficient counterparts (19D or 49D) were subjected to osmotic (1, 2, and 3 M NaCl) or chlorine (25, 50, and 100 μg/ml sodium hypochlorite) treatments. The survival of STEC cells was determined at different treatment intervals. Populations of 19B cells were significantly higher (P < 0.05) than those of 19D cells at all sampling intervals for the chlorine treatments, at 24- to 48-h intervals for the 1 M NaCl treatment, and at 9- to 48-h intervals for the 2 M NaCl treatment. Significant differences in populations of 49B and 49D cells were observed after 9, 36, and 48 h of treatment with 2 M NaCl and after 3, 12, 36, and 48 h of treatment with 3 M NaCl (P < 0.05). Populations of 49B cells were higher than those of 49D cells (P < 0.05) also after 5 to 10 min of treatment with 50 μg/ml sodium hypochlorite and 3 to 10 min of treatment with 100 μg/ml sodium hypochlorite. The protective effects conferred by cellulose may explain the greater survival of cellulose-producing STEC under adverse environmental conditions.
Document Type: Research Article
Affiliations: Department of Food Science and Technology, University of Georgia, Griffin, Georgia 30223, USA
Publication date: 2010-11-01
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