Quantitative PCR Method for Evaluating Freshness of Whiting (Merlangius merlangus) and Plaice (Pleuronectes platessa)

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Abstract:

We have developed a method for rapid quantification of fish spoilage bacteria based on quantitative PCR with degenerated oligonucleotides that hybridize on the torA gene coding for trimethylamine N-oxide reductase, one of the major bacterial enzymes in fish spoilage. To show the utility of this gene, we used a regular PCR with DNA extracts from whiting (Merlangius merlangus) and plaice (Pleuronectes platessa) stored in ice. Quantitative PCR showed that the number of copies of the torA gene, i.e., the number of spoilage bacteria, increases with length of storage. This approach can therefore be used to evaluate freshness for the two fish species studied (whiting and plaice).

Document Type: Research Article

Affiliations: 1: Agence Française de Sécurité Sanitaire des Aliments (AFSSA), Laboratoire d'Études et de Recherches sur les Produits de la Pêche, Boulevard Bassin Napoléon, 62200 Boulogne sur Mer, France. g.duflos@afssa.fr 2: Laboratoire de Chimie Bactérienne, UPR 9043, CNRS, Aix-Marseille University, 31, Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France 3: Agence Française de Sécurité Sanitaire des Aliments (AFSSA), Laboratoire d'Études et de Recherches sur les Produits de la Pêche, Boulevard Bassin Napoléon, 62200 Boulogne sur Mer, France

Publication date: July 1, 2010

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