Frequency of Toxoplasma gondii in Pork Meat in Ocotlán, Jalisco, Mexico
Abstract:Toxoplasmosis is an infection caused by Toxoplasma gondii, an intracellular obligate parasite. Its transmission has usually been attributed to ingestion of undercooked or raw meat. The frequency of T. gondii in pork, the most common meat for human consumption in Jalisco, Mexico, is unknown; in Guadalajara city high prevalence of human toxoplasmosis has been documented. Forty-eight samples of pork meat from butcher shops in Ocotlán city were analyzed. Through bioassay, 50 g of tissue was homogenized in an acidic pepsin solution and inoculated subcutaneously to previously immunosuppressed mice. Blood samples from the mice tail vein were obtained before inoculation and 7, 14, 28, and 45 days postinoculation to analyze anti-Toxoplasma immunoglobulin (Ig) M and IgG antibody kinetics by indirect enzyme-linked immunosorbent assay. For histopathology, small fragments of the brain, lungs, heart, and skeletal muscle were extracted on day 45 and were stained with hematoxylin and eosin. Also, DNA was extracted from the pork meat for PCR amplification of the B1 gene. Even though all pork samples were negative by histopathology and PCR, IgG and IgM antibodies against T. gondii were detected in 1 of the 48 inoculated mice, reflecting a frequency of 2.1% positive pork meat, which is lower than expected but similar to that found in other regions.
Document Type: Research Article
Affiliations: 1: Laboratorio de Neurofisiología, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Sierra Mojada 950, Col. Independencia, Guadalajara, Jalisco, México 2: Departamento de Ciencias Básicas, Centro Universitario de la Ciénega, Universidad de Guadalajara. Av. Universidad 1115 C.P. 47810, Col. Lindavista, Ocotlán, Jalisco, México 3: Laboratorio de Inmunología Experimental Instituto Nacional de Pediatría, SSA, México Insurgentes Sur 3700 C, Col. Insurgentes Cuicuilco, Delegación Coyoacán C.P. 04530, México, D.F. 4: Laboratorio de Patología del Hospital Civil "Juan I Menchaca," Salvador Quevedo y Zubieta No. 750, C.P. 44340, Guadalajara, Jalisco, México 5: Instituto de Enfermedades Crónico Degenerativas, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Sierra Mojada 950, Col. Independencia, Guadalajara, Jalisco, México 6: Instituto de Biología Molecular y Terapia Génica, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Sierra Mojada 950, Col. Independencia, Guadalajara, Jalisco, México
Publication date: June 1, 2010
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