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Maturation and Survival of Cronobacter Biofilms on Silicone, Polycarbonate, and Stainless Steel after UV Light and Ethanol Immersion Treatments

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Cronobacter sakazakii cells in biofilms formed on silicone, polycarbonate, and stainless steel coupons immersed in reconstituted powdered infant milk formula were treated with ethanol (10 to 70%) and UV light (12 to 2,160 mW·s/cm2) as antibacterial treatments. Biofilm maturation curves were determined after immersion at 25°C for up to 144 h. Populations increased after subsequent immersion at 25°C for 24 h in reconstituted powdered infant milk formula to the respective maximum levels of 7.96, 7.91, and 6.99 log CFU per coupon. Populations attached to silicone and polycarbonate surfaces to a greater extent than to stainless steel (P < 0.05). Treatment with 10% ethanol did not cause a significant decrease in the level of C. sakazakii, but treatment with 30, 40, and 50% ethanol reduced the levels to approximately 1.73, 3.02, and 4.17 log CFU per coupon, respectively. C. sakazakii was not detected on any coupon after treatment with 70% ethanol or 2,160 mW·s/cm2 UV light. A synergistic effect of sequential ethanol and UV treatments was not observed.

Document Type: Research Article

Affiliations: 1: Department of Food Science and Technology, Chung-Ang University, 72-1 Naeri, Ansung, Gyunggi-do 456-756, South Korea 2: Department of Food Science and Technology, Chung-Ang University, 72-1 Naeri, Ansung, Gyunggi-do 456-756, South Korea;, Email:

Publication date: May 1, 2010

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