Survival of Enterococcus faecalis OG1RF:pCF10 in Poultry and Cattle Feed: Vector Competence of the Red Flour Beetle, Tribolium castaneum (Herbst)

$37.00 plus tax (Refund Policy)

Buy Article:


Laboratory experiments were designed to determine the survival of Enterococcus faecalis OG1RF:pCF10 in poultry and cattle feed and its acquisition and transmission by adults of the red flour beetle, Tribolium castaneum (Herbst), to sterile feed. Adult T. castaneum beetles were introduced into poultry and cattle feed inoculated with E. faecalis OG1RF:pCF10 and incubated at 28°C with 65% relative humidity for 7 days in a growth chamber. E. faecalis survived in both poultry and cattle feed during the 7-day test period. There was a logarithmic decrease in E. faecalis concentration in poultry and cattle feed and in and on the insects. E. faecalis persisted on the surface and within T. castaneum adults for 7 days when adults were released on E. faecalis–inoculated poultry feed and for only 5 days on E. faecalis–inoculated cattle feed. The concentration of E. faecalis decreased more slowly on poultry feed than on cattle feed, and this may explain why adult T. castaneum insects were more successful in acquiring and transferring E. faecalis from inoculated poultry feed to sterile poultry feed during the 7-day test period. However, T. castaneum adults reared on inoculated cattle feed were unable to contaminate sterile cattle feed on day 7. To our knowledge, this is the first report documenting T. castaneum to successfully acquire antibiotic-resistant enterococci from animal feed and transfer them to sterile feed. Management of T. castaneum through effective integrated pest management program is therefore important to prevent the spread of antibiotic-resistant and virulent enterococci in animal feed and feed manufacturing environments.

Document Type: Research Article

Affiliations: 1: Department of Grain Science and Industry, Kansas State University, Manhattan, Kansas 66506-2201, USA 2: Department of Grain Science and Industry, Kansas State University, Manhattan, Kansas 66506-2201, USA; Email: 3: Department of Entomology, Diagnostic Medicine and Pathobiology, Kansas State University, Manhattan, Kansas 66506-2201, USA

Publication date: March 1, 2010

More about this publication?
  • IAFP members must first sign in on the right to access full text articles of JFP

    First published in 1937, the Journal of Food Protection®, is a refereed monthly publication. Each issue contains scientific research and authoritative review articles reporting on a variety of topics in food science pertaining to food safety and quality. The Journal is internationally recognized as the leading publication in the field of food microbiology with a readership exceeding 11,000 scientists from 70 countries. The Journal of Food Protection® is indexed in Index Medicus, Current Contents, BIOSIS, PubMed, Medline, and many others.

    Print and online subscriptions are available to Members and Institutional subscribers. Online visitors who are not IAFP Members or journal subscribers will be charged on a pay-per-view basis. Information can be obtained by calling +1 800.369.6337; +1 515.276.3344; fax: +1 515.276.8655, E-mail: or Web site:
  • Information for Authors
  • Submit a Paper
  • Subscribe to this Title
  • Membership Information
  • Information for Advertisers
  • ingentaconnect is not responsible for the content or availability of external websites
Related content



Share Content

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more