Detection of Allergen Walnut Component in Food by an Improved Real-Time PCR Method

Authors: Wang, Haiyan¹; Yuan, Fei²; Wu, Yajun²; Yang, Hairong²; Xu, Baoliang²; Liu, Zhongxue³; Chen, Ying⁴

Source: Journal of Food Protection®, Volume 72, Number 11, November 2009 , pp. 2433-2435(3)

Publisher: International Association for Food Protection

Buy & download fulltext article:

Price: $37.00 plus tax (Refund Policy)

Abstract:

A real-time PCR method aimed at the gene sequence of the walnut vicilin-like seed storage protein was established for the detection of the allergen walnut in food. The primers and probe were designed based on published methods. The method provided positive results for walnut and negative results for other tested agricultural plant materials including pecan. The intrinsic detection limit of the method was 0.00125 ng of walnut DNA, and the practical detection limit was 0.001% (wt/wt) walnut content in wheat; both of these values are lower than that of previously published methods. Therefore, this real-time PCR method is sufficiently specific and sensitive for the detection of walnut component in food.

Document Type: Research article

Affiliations: 1: Institute of Food Safety, Chinese Academy of Inspection and Quarantine, No. 3, Gaobeidian North Road, Chaoyang District, Beijing 100123, People's Republic of China; Inner Mongolia Entry-Exit Inspection and Quarantine Bureau, No. 12, Erdos Street, 2: Institute of Food Safety, Chinese Academy of Inspection and Quarantine, No. 3, Gaobeidian North Road, Chaoyang District, Beijing 100123, People's Republic of China 3: Inner Mongolia Entry-Exit Inspection and Quarantine Bureau, No. 12, Erdos Street, Huhhot, Inner Mongolia 010020, People's Republic of China 4: Institute of Food Safety, Chinese Academy of Inspection and Quarantine, No. 3, Gaobeidian North Road, Chaoyang District, Beijing 100123, People's Republic of China;, Email: yqychen@yahoo.com.cn

Publication date: 2009-11-01

More about this publication?

IAFP members must first sign in on the right to access full text articles of JFP

First published in 1937, the Journal of Food Protection^®, is a refereed monthly publication. Each issue contains scientific research and authoritative review articles reporting on a variety of topics in food science pertaining to food safety and quality. The Journal is internationally recognized as the leading publication in the field of food microbiology with a readership exceeding 11,000 scientists from 70 countries. The Journal of Food Protection^® is indexed in Index Medicus, Current Contents, BIOSIS, PubMed, Medline, and many others.

Print and online subscriptions are available to Members and Institutional subscribers. Online visitors who are not IAFP Members or journal subscribers will be charged on a pay-per-view basis. Information can be obtained by calling +1 800.369.6337; +1 515.276.3344; fax: +1 515.276.8655, E-mail: info@foodprotection.org or Web site: www.foodprotection.org
Information for Authors
Submit a Paper
Subscribe to this Title
Membership Information
Information for Advertisers
ingentaconnect is not responsible for the content or availability of external websites

Detection of Allergen Walnut Component in Food by an Improved Real-Time PCR Method

Buy & download fulltext article:

Tools

Key

Text size:

Useful pages