IngentaConnect Neonatal Mice as Models for Cronobacter sakazakii Infection in In...

Authors: Richardson, Arena N.¹; Lambert, Sonya²; Smith, Mary Alice³

Source: Journal of Food Protection®, Volume 72, Number 11, November 2009 , pp. 2363-2367(5)

Publisher: International Association for Food Protection

Abstract:

Cronobacter sakazakii is an opportunistic pathogen that has been isolated from powdered infant formulas. C. sakazakii infection can result in serious illnesses such as bacteremia, septicemia, meningitis, and death in at-risk infants who are orally fed contaminated reconstituted powdered infant formulas. The objective of this study was to compare the susceptibilities of BALB/c, C57BL/6, and CD-1 mice to C. sakazakii strain MNW2. We acquired timed-pregnant CD-1 mice and allowed them to give birth naturally. On postnatal day 3.5, each pup was administered a total dose of approximately 10² to 10¹¹ CFU C. sakazakii strain MNW2 in reconstituted powdered infant formula. Mice were observed twice daily for morbidity and mortality. At postnatal day 10.5, the remaining pups were euthanized, and brain, liver, and cecum were excised and analyzed for the presence of C. sakazakii. C. sakazakii was isolated from brains, livers, and ceca in all three mouse strains. The CD-1 mouse strain was the most susceptible of the three, with the lowest infectious dose (10² CFU) and the lowest lethal dose (also 10² CFU).

Document Type: Research article

Affiliations: 1: Department of Environmental Health Science, 206 Environmental Health Science Building, University of Georgia, Athens, Georgia 30602-2102 2: Department of Environmental Health Science, 206 Environmental Health Science Building, University of Georgia, Athens, Georgia 30602-2102, Center for Food Safety, 1109 Experiment Street, Melton Building, Griffin, Georgia 30223-1797, USA 3: Department of Environmental Health Science, 206 Environmental Health Science Building, University of Georgia, Athens, Georgia 30602-2102, Center for Food Safety, 1109 Experiment Street, Melton Building, Griffin, Georgia 30223-1797, USA;, Email: m

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Neonatal Mice as Models for Cronobacter sakazakii Infection in Infants