Skip to main content

Biological Control of Aflatoxin Contamination in Corn Using a Nontoxigenic Strain of Aspergillus flavus

Buy Article:

$37.00 plus tax (Refund Policy)


A 2-year study was conducted to determine the efficacy of different applications of a nontoxigenic strain of Aspergillus flavus for reducing aflatoxin contamination in corn. Treatments consisted of the nontoxigenic strain in the form of (i) conidia-coated hulled barley applied to soil when corn was about 0.8 m tall, (ii) conidia-coated hulled barley applied in plant whorls prior to tasseling, (iii) multiple applications of a spray formulation of conidia during silking, and (iv) untreated control. Treatments were replicated eight times in individual plots consisting of four rows of 18 m each. In year 1, no significant differences were associated with treatments for aflatoxin, total A. flavus colonization, or incidence of nontoxigenic isolates of A. flavus in corn, which were all relatively high, ranging from 83.8 to 93.1%. In year 2, the whorl application produced a significantly lower mean aflatoxin concentration of 49.5 ppb compared with all other treatments, while both the soil (108.3 ppb) and spray applications (173.7 ppb) were significantly reduced compared with the control (191.6 ppb). The whorl application was the only treatment that had a significantly higher incidence (86.5%) of nontoxigenic isolates of A. flavus than the control had, which was still relatively high at 69.1%. Data indicated that applications of the nontoxigenic strain influenced untreated corn, thus reducing the apparent effect of the biocontrol treatments. Larger-scale studies with greater separation between treated and untreated fields are warranted.

Document Type: Research Article

Affiliations: U.S. Department of Agriculture, Agricultural Research Station, National Peanut Research Laboratory, 1011 Forrester Drive S.E., Dawson, Georgia 39842, USA

Publication date: April 1, 2009

More about this publication?
  • IAFP Members with personal subscriptions to JFP Online: To access full-text JFP or JMFT articles, you must sign-in in the upper-right corner using your Ingenta sign-in details (your IAFP Member Login does not apply to this website).

    The Journal of Food Protection (JFP) is a refereed monthly publication. Each issue contains scientific research and authoritative review articles reporting on a variety of topics in food science pertaining to food safety and quality. The Journal is internationally recognized as the leading publication in the field of food microbiology with a readership exceeding 11,000 scientists from 70 countries. The Journal of Food Protection is indexed in Index Medicus, Current Contents, BIOSIS, PubMed, Medline, and many others.

    Print and online subscriptions are available to IAFP Members and institutional subscribers. IAFP Members with a subscription to JFP Online will have access to all available JFP and JMFT content. Online visitors who are not IAFP Members or journal subscribers will be charged on a pay-per-view basis. Membership and subscription information is available at
  • Information for Authors
  • Submit a Paper
  • Subscribe to this Title
  • Membership Information
  • Information for Advertisers
  • Ingenta Connect is not responsible for the content or availability of external websites

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more