Bactericidal Activity of Lactoferrin and Its Amidated and Pepsin-Digested Derivatives against Pseudomonas fluorescens in Ground Beef and Meat Fractions
Abstract:The antibacterial activity of lactoferrin (LF) and its amidated and pepsin-digested derivatives (AMILF and PDLF, respectively) against Pseudomonas fluorescens ATCC 948 in ground beef was investigated. LF, AMILF, and PDLF at 1 mg/ml decreased bacterial counts by 1.9, 6.4, and 3.5 log units, respectively, after 1 h at 30°C when the assays were performed in distilled water, but their bactericidal activity disappeared when added at 1 mg/g to ground beef held for 24 h at 5°C. To identify meat components responsible for the loss of bactericidal activity, ground beef was homogenized and separated into fractions of different molecular weights. When cations were removed (fraction > 1 kDa), the bactericidal activity of AMILF was completely restored, whereas the effectiveness of LF and PDLF remained 1.0 and 0.4 log units lower, respectively, than the results obtained in distilled water. EDTA at 5 mM greatly enhanced the bactericidal activity of the three antimicrobials at 1 mg/ml in meat homogenate and in the presence of 5 mM sodium bicarbonate completely restored the bactericidal activity. However, when 1 mg/g AMILF, 5 mM sodium bicarbonate, and increasing EDTA concentrations were added to inoculated ground beef, bacterial counts declined by only 0.2, 0.4, and 1.2 log units in the presence of 8, 32, and 128 mM EDTA, respectively, after 24 h at 5°C.
Document Type: Research Article
Affiliations: Departamento de Tecnología de Alimentos, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Carretera de la Coruña Km 7, Madrid 28040, Spain
Publication date: 2009-04-01
More about this publication?
- The Journal of Food Protection has moved to a new website. Please use http://jfoodprotection.org to access the Journal of Food Protection and Journal of Milk & Food Technology content on the new JFP site. Content on the IngentaConnect website will not be available after December 31, 2016.
- Information for Authors
- Submit a Paper
- Subscribe to this Title
- Membership Information
- Information for Advertisers
- Ingenta Connect is not responsible for the content or availability of external websites