Evaluation of Three Commercially Available Enzyme-Linked Immunosorbent Assay Kits for Detection of Shiga Toxin

Authors: Willford, John1; Mills, Kenneth2; Goodridge, Lawrence D.3

Source: Journal of Food Protection®, Number 4, April 2009, pp. 696-914 , pp. 741-747(7)

Publisher: International Association for Food Protection

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Three commercially available Shiga toxin (Stx) enzyme-linked immunosorbent assay (ELISA) kits were evaluated for their ability to detect Stx in pure cultures of Stx-producing Escherichia coli (specificity). The detection limits (sensitivity) of each ELISA kit were also evaluated. Seventy-eight Stx-producing E. coli (STEC) isolates that produced Stx1, Stx2, or Stx1 and Stx2 variants were examined in this study. The specificities of the tests were comparable, and the sensitivities of two of the tests (Premier EHEC and rBiopharm Ridascreen Verotoxin Enzyme Immunoassay) were within the same order of magnitude. The ProSpecT Shiga Toxin E. coli Microplate Assay was approximately 10-fold less sensitive. The inability of all three tests to detect the Stx2d and Stx2e variants indicated that some STEC strains may not be detected by Stx ELISA. The ability of the Premier EHEC ELISA to detect toxin in artificially inoculated bovine fecal samples (following enrichment) indicated that this kit may be used to screen cattle for the presence of Stx as an indicator of the presence of STEC. In particular, such a screening method could be useful during the summer, when the number of STEC-positive animals and the number of STEC that they shed increase.

Document Type: Research Article

Affiliations: 1: Department of Animal Science, Department 3684, University of Wyoming, 1000 East University Avenue, Laramie, Wyoming 82071 2: Department of Veterinary Sciences, Department 22150, University of Wyoming, 1000 East University Avenue, Laramie, Wyoming 82071 3: Department of Animal Sciences, Colorado State University, 350 West Pitkin Street, Fort Collins, Colorado 80523, USA

Publication date: April 1, 2009

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