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Escherichia coli O157:H7 Lineages in Healthy Beef and Dairy Cattle and Clinical Human Cases in Alberta, Canada

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Abstract:

The aim of this study was to examine the prevalence and distribution of Escherichia coli O157:H7 lineage-specific polymorphism assay (LSPA) 6 genotypes from cattle (n = 313) and clinical human (n = 203) isolates from northern and southern Alberta, Canada, to understand possible associations of genotypes with host and geographic location. The majority of cattle isolates (feedlot and dairy) typed as LSPA-6 111111 (72.2%), with proportionately higher LSPA-6 222222 (19.4%) than other LSPA-6 genotypes (10.7%). Clinical human isolates also typed primarily as LSPA-6 111111 (90.1%), but a higher percentage of genotypes (6.8%) other than LSPA-6 222222 (3.1%) was observed. A significantly higher frequency of LSPA-6 111111 in southern Alberta cattle (P < 0.0001) and a significant difference in LSPA-6 genotypes between human versus feedlot cattle from northern Alberta (P < 0.0001) were detected. LSPA-6 211111 genotype was third and second most common in cattle and humans, respectively, and several new LSPA-6 genotypes (n = 19) were also discovered. Despite avoiding over-representation of isolates from specific farms or outbreaks, higher strain diversity among cattle by pulsed-field gel electrophoresis (PFGE; 50 genotypes) in contrast to human (9 PFGE genotypes) isolates was observed. The majority of cattle (74.4%) and human (90.6%) isolates were susceptible to the antimicrobials tested. Within resistant cattle isolates, sulfisoxazole-tetra-cycline resistance was common (62.5%) and was accounted for by the presence of sul1 and sul2, and tet(A) and tet(B) determinants. An association between LSPA-6 and PFGE genotypes but not between geographic location and PFGE genotype for both hosts was evident.

Document Type: Research Article

Affiliations: 1: Agriculture and Agri-Food Research Centre, P.O. Box 3000, Lethbridge, Alberta, Canada T1J 4B1;, Email: sharmar@agr.gc.ca 2: Alberta Agriculture and Food, 5403 1st Avenue S., Lethbridge, Alberta, Canada T1J 4V6 3: Faculty of Medicine, Department of Microbiology and Infectious Diseases, Provlab, 3030 Hospital Drive N.W., Calgary, Alberta, Canada T2N 4N1 4: Agriculture and Agri-Food Research Centre, P.O. Box 3000, Lethbridge, Alberta, Canada T1J 4B1 5: Laboratory for Foodborne Zoonoses, Public Health Agency of Canada, CFIA, Lethbridge, Alberta, Canada T1J 3Z4 6: Provincial Laboratory for Public Health (Microbiology), Edmonton, Alberta, Canada T6G 2J2 7: Faculty of Medicine, Department of Microbiology and Infectious Diseases, Peter Lougheed Centre, 3500-26 Avenue N.E., Calgary, Alberta, Canada T1Y 6J4 8: Agriculture and Agri-Food Research Centre, London, Ontario, Canada N5V 4T3

Publication date: March 1, 2009

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