Immunocapture and Real-Time PCR To Detect Campylobacter spp.

Authors: Morales-Rayas, Rocío¹; Wolffs, Petra F.G.²; Griffiths, Mansel W.¹

Source: Journal of Food Protection®, Volume 71, Number 12, December 2008 , pp. 2543-2547(5)

Publisher: International Association for Food Protection

Purchase options

The full text electronic article is available for purchase. You will be able to download the full text electronic article after payment.

$37.00 plus tax Refund Policy

More about this publication?

First published in 1937, the Journal of Food Protection^®, is a refereed monthly publication. Each issue contains scientific research and authoritative review articles reporting on a variety of topics in food science pertaining to food safety and quality. The Journal is internationally recognized as the leading publication in the field of food microbiology with a readership exceeding 11,000 scientists from 70 countries. The Journal of Food Protection^® is indexed in Index Medicus, Current Contents, BIOSIS, PubMed, Medline, and many others.

Print and online subscriptions are available to Members and Institutional subscribers. Online visitors who are not IAFP Members or journal subscribers will be charged on a pay-per-view basis.

Information can be obtained by calling +1 800.369.6337; +1 515.276.3344; fax: +1 515.276.8655, E-mail: info@foodprotection.org or Web site: www.foodprotection.org
Information for Authors
Submit a Paper
Subscribe to this Title
Membership Information
Information for Advertisers

More like this?

In this: publication
By this: publisher
In this Subject: Nutrition & Food
By this author: Morales-Rayas, Rocío ; Wolffs, Petra F.G. ; Griffiths, Mansel W.

Content Key:

- Free

- New

- Open Access

- Subscribed

- Free Trial

Abstract:

In this work, the feasibility of using a large-volume immunocapture system as a sample pretreatment before detection of Campylobacter was studied. Real-time PCR was used for detection of captured cells after immunocapture. This immunocapture system is able to process high-volume samples by recirculation, increasing the possibility of capturing cells in low numbers. After 30 min of recirculation, the sample is concentrated from 250 ml to 200 μl. In this study, different parameters were compared in order to improve cell capture. The analysis of inoculated chicken skin showed that detection of Campylobacter at levels of 10³ CFU/25 g was possible after 8 h of enrichment. The low recovery of Campylobacter cells (<1%) makes this separation method qualitative rather than quantitative. The detection limit of the entire protocol was increased due to the low cell recovery of the sample pretreatment. Therefore, this immunoseparation is able to recover cells present in high concentration after enrichment but not cells present in low concentration. Isolation of Campylobacter cells is achievable using this separation method rather than rapid detection.

Document Type: Research article

Affiliations: 1: Department of Food Science, University of Guelph, Guelph, Ontario, Canada N1G 2W1; Canadian Research Institute for Food Safety, 43 McGilvray Street, Guelph, Ontario, Canada N1G 2W1 2: Canadian Research Institute for Food Safety, 43 McGilvray Street, Guelph, Ontario, Canada N1G 2W1; Department of Medical Microbiology, University Hospital of Maastricht, Peter Debyelaan 25, P.O. Box 5800, 6202 AZ, Maastricht, The Netherlands