Comparison of the BAX System PCR Method to Brazil's Official Method for the Detection of Salmonella in Food, Water, and Environmental Samples
Abstract:A two-stage study compared the BAX system PCR method with the reference culture method used by the Brazilian Ministry of Agriculture and Food Supply for the detection of Salmonella in food, water, and environmental samples. In stage 1, fish matrix samples (n = 258) were spiked at several levels with Salmonella and a combination of Salmonella and non-Salmonella competitive organisms. Replicates were analyzed by the BAX system PCR method and the reference method with comparable results (sensitivity ≥ 97.5%, specificity ≥ 83.3%) from both methods at the limit of detection. In stage 2, a total of 1,988 samples with 70 product types were analyzed with both methods. Five laboratories were involved in this study, and the samples used were from routine analyses. The BAX system PCR method was shown to be comparable to the reference method, with a limit of detection of 1.0 to 2.0 CFU/25 g of sample. Analysis of the results obtained in stage 2 and in the combination of stages 1 and 2 for the BAX system showed the following performance: sensitivity ≥ 99.0%, specificity ≥ 97.2%, false-negative rate ≤ 1.1%, and false-positive rate ≤ 2.8%. Therefore, the BAX system appears to be equivalent to the reference method, with ≥97.3% agreement.
Document Type: Research Article
Affiliations: 1: National Industrial Apprenticeship Service (SENAI), Food Technology Center (LANAL)–Microbiology, Rua Frei Bruno, 201E-Bairro Jardim América, CEP 89.803-800, Chapecó, Santa Catarina, Brazil 2: Ministry of Agriculture and Food Supply–Animal Laboratory Coordination, Brazil, Distrito Federal, Brazil 3: Ministry of Agriculture and Food Supply (LAPA/PE), Recife, Pernambuco, Brazil 4: Ministry of Agriculture and Food Supply (LARA/SP), Campinas, São Paulo, Brazil 5: Food Intelligence (FI), São Paulo, São Paulo, Brazil 6: Products Analysis Laboratory (SFDK), São Paulo, São Paulo, Brazil 7: Madasa, Brasil 8: Epagri, Chapecó, Santa Catarina, Brazil 9: Embrapa, Concórdia, Santa Catarina, Brazil
Publication date: December 1, 2008
- IAFP Members with personal subscriptions to JFP Online: To access full-text JFP or JMFT articles, you must sign-in in the upper-right corner using your Ingenta sign-in details (your IAFP Member Login does not apply to this website). The Journal of Food Protection (JFP) is a refereed monthly publication. Each issue contains scientific research and authoritative review articles reporting on a variety of topics in food science pertaining to food safety and quality. The Journal is internationally recognized as the leading publication in the field of food microbiology with a readership exceeding 11,000 scientists from 70 countries. The Journal of Food Protection is indexed in Index Medicus, Current Contents, BIOSIS, PubMed, Medline, and many others.
Print and online subscriptions are available to IAFP Members and institutional subscribers. IAFP Members with a subscription to JFP Online will have access to all available JFP and JMFT content. Online visitors who are not IAFP Members or journal subscribers will be charged on a pay-per-view basis. Membership and subscription information is available at www.foodprotection.org.
- Information for Authors
- Submit a Paper
- Subscribe to this Title
- Membership Information
- Information for Advertisers
- ingentaconnect is not responsible for the content or availability of external websites