A Rapid High-Performance Liquid Chromatography with Fluorescence Detection Method Developed To Analyze Ochratoxin A in Wine
Authors: Tafuri, Alessio; Meca, Giuseppe; Ritieni, Alberto
Source: Journal of Food Protection®, Number 10, October 2008, pp. 1960-2160 , pp. 2133-2137(5)
Abstract:A rapid, sensitive, reproducible, and inexpensive method of high-performance liquid chromatography with fluorescence detection (HPLC-FLD) for the analysis of ochratoxin A (OTA) in wine was developed. It is characterized by direct injection of the wine into the HPLC apparatus, with no need of extraction or cleanup. The method uses acetonitrile, water, and acetic acid (49:49:2, vol/vol/vol, respectively) as the isocratic mobile phase and a 5-μm monolithic C18 column (100 by 3 mm inside diameter). The relative standard deviation obtained in the OTA determination varied between 0.22 and 1.76%, with a mean value of 0.89%, in samples with concentrations between 0.10 and 100 ng/ml. The recovery of OTA ranged from 102% in samples spiked with 1 ng/ml OTA to 120% in samples with 0.10 ng/ml OTA. The method compared favorably with a published method based on an immunoaffinity column cleanup and a chromatographic assay with a C18 conventional HPLC column.
Document Type: Research Article
Affiliations: Dipartimento di Scienza degli Alimenti, Università di Napoli "Federico II," via Università n° 100, Parco Gussone, 80055, Portici, Napoli, Italy
Publication date: October 1, 2008
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