Shopping cart
Associations between the Presence and Magnitude of Escherichia coli O157 in Feces at Harvest and Contamination of Preintervention Beef Carcasses
Authors: Fox, J.T.1; Renter, D.G.1; Sanderson, M.W.2; Nutsch, A.L.3; Shi, X.1; Nagaraja, T.G.1
Source: Journal of Food Protection®, Volume 71, Number 9, September 2008 , pp. 1761-1767(7)
Abstract:
To quantify associations at slaughter between Escherichia coli O157 carcass contamination, fecal-positive animals, and high-shedding animals within truckloads of finished cattle, we sampled up to 32 cattle from each of 50 truckloads arriving at a commercial abattoir in the Midwest United States during a 5-week summer period. Carcass swab samples collected preevisceration and fecal samples collected postevisceration were matched within animals and analyzed for the presence of E. coli O157, using enrichment, immunomagnetic separation, and plating on selective media (IMS). In addition, a direct plating procedure was performed on feces to identify high-shedding animals. E. coli O157 was isolated from 39 (2.6%) of 1,503 carcass samples in 15 (30%) truckloads, and 127 (8.5%) of 1,495 fecal samples in 37 (74%) truckloads. Fifty-five (3.7%) high-shedding animals were detected from 26 (52%) truckloads. Truckload high-shedder (Spearman rank-order correlation coefficient [rs ] 0.68), IMS-positive (rs 0.48), and combined fecal (rs 0.61) prevalence were significantly correlated with carcass prevalence. The probability of isolating E. coli O157 from a carcass was not significantly associated with the high-shedder or fecal IMS status of the animal from which the carcass was derived. However, the probability of carcass contamination was significantly associated with all truckload-level measures of fecal E. coli O157, particularly whether or not a high shedder was present within the truckload (odds ratio 16.2; 95% confidence interval, 6.3-43.6). Our results suggest that high shedders within a truckload at slaughter could be a target for mitigation strategies to reduce the probability of preevisceration carcass contamination.Document Type: Research article
Affiliations: 1: Department of Diagnostic Medicine, Food Science Institute, Kansas State University, Manhattan, Kansas 66506-5606, USA 2: Department of Clinical Sciences, College of Veterinary Medicine, Food Science Institute, Kansas State University, Manhattan, Kansas 66506-5606, USA 3: Department of Animal Sciences and Industry, Food Science Institute, Kansas State University, Manhattan, Kansas 66506-5606, USA
Publication date: 2008-09-01
- IAFP members must first sign in on the right to access full text articles of JFP First published in 1937, the Journal of Food Protection®, is a refereed monthly publication. Each issue contains scientific research and authoritative review articles reporting on a variety of topics in food science pertaining to food safety and quality. The Journal is internationally recognized as the leading publication in the field of food microbiology with a readership exceeding 11,000 scientists from 70 countries. The Journal of Food Protection® is indexed in Index Medicus, Current Contents, BIOSIS, PubMed, Medline, and many others.
Print and online subscriptions are available to Members and Institutional subscribers. Online visitors who are not IAFP Members or journal subscribers will be charged on a pay-per-view basis. Information can be obtained by calling +1 800.369.6337; +1 515.276.3344; fax: +1 515.276.8655, E-mail: info@foodprotection.org or Web site: www.foodprotection.org - Information for Authors
- Submit a Paper
- Subscribe to this Title
- Membership Information
- Information for Advertisers
- ingentaconnect is not responsible for the content or availability of external websites
- In this: publication
- By this: publisher
- In this Subject: Nutrition & Food
- By this author: Fox, J.T. ; Renter, D.G. ; Sanderson, M.W. ; Nutsch, A.L. ; Shi, X. ; Nagaraja, T.G.
Tools
Receive new issue alert
Latest TOC RSS Feed
Get PermissionsKey
Print this page