Evaluation of an Automated Repetitive Sequence-Based PCR System for Subtyping Enterobacter sakazakii
Authors: Healy, B.1; Mullane, N.1; Collin, V.2; Mailler, S.2; Iversen, C.3; Chatellier, S.2; Storrs, M.4; Fanning, S.1
Source: Journal of Food Protection®, Volume 71, Number 7, July 2008 , pp. 1372-1378(7)
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Abstract:
Enterobacter sakazakii is regarded as a ubiquitous organism that can be isolated from a wide range of foods and environments. Infection in at-risk infants has been epidemiologically linked to the consumption of contaminated powdered infant formula. Preventing the dissemination of this pathogen in a powdered infant formula manufacturing facility is an important step in ensuring consumer confidence in a given brand together with the protection of the health status of a vulnerable population. In this study we report the application of a repetitive sequence-based PCR typing method to subtype a previously well-characterized collection of E. sakazakii isolates of diverse origin. While both methods successfully discriminated between the collection of isolates, repetitive sequence-based PCR identified 65 types, whereas pulsed-field gel electrophoresis identified 110 types showing ≥95% similarity. The method was quick and easy to perform, and our data demonstrated the utility and value of this approach to monitor in-process contamination, which could potentially contribute to a reduction in the transmission of E. sakazakii.Document Type: Research article
Affiliations: 1: Centre for Food Safety, School of Agriculture, Food Science and Veterinary Medicine, University College Dublin, Belfield, Dublin 4, Ireland 2: bioMérieux, La Balme Les Grottes, France 3: Institute for Food Safety & Hygiene, Vetsuisse Faculty, University of Zurich, CH-8057 Zurich, Switzerland 4: bioMérieux, Marcy l'Etoile, France
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