Characterization of a Second Ornithine Decarboxylase Isolated from Morganella morganii

Authors: De Las Rivas, Blanca; González, Ramón; Landete, José María; Muñoz, Rosario

Source: Journal of Food Protection®, Volume 71, Number 3, March 2008 , pp. 657-661(5)

Publisher: International Association for Food Protection

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Abstract:

The genes involved in the putrescine formation by Morganella morganii were investigated because putrescine is an indicator of food process deterioration. We report here on the existence of a new gene for ornithine decarboxylase (ODC) in M. morganii. The sequenced 5,311-bp DNA region showed the presence of four complete and one partial open reading frame. Putative functions have been assigned to several gene products by sequence comparison with the proteins included in the databases. The third open reading frame (speC) encoded a 722-amino acid protein showing 70.9% identity to the M. morganii ODC previously characterized (SpeF). The speC gene has been expressed in Escherichia coli, resulting in ODC activity. The presence of a functional promoter (PspeC) located upstream of speC has been demonstrated. Quantitative real-time reverse transcription PCR assay was used to quantify expression of both M. morganii ODC-encoding genes, speC and speF, under different growth conditions. This assay allows us to identify SpeF as the inducible M. morganii ODC, since it was highly expressed in the presence of ornithine.

Document Type: Research article

Affiliations: 1: Departamento de Microbiología, Instituto de Fermentaciones Industriales, CSIC, Juan de la Cierva 3, 28006 Madrid, Spain

Publication date: 2008-03-01

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Characterization of a Second Ornithine Decarboxylase Isolated from Morganella morganii

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