Growth Response of Salmonella Typhimurium in the Presence of Natural and Synthetic Antimicrobials: Estimation of MICs from Three Different Models

Authors: Guillier, L.¹; Nazer, A.I.²; Dubois-Brissonnet, F.³

Source: Journal of Food Protection®, Volume 70, Number 10, October 2007 , pp. 2243-2250(8)

Publisher: International Association for Food Protection

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Abstract:

The aim of this study was to determine the MICs of 14 antimicrobials for Salmonella Typhimurium with three methods and to check the influence of experiment duration on the estimation of MICs. The growth of Salmonella Typhimurium in a brain heart infusion medium containing various concentrations of natural aromatic compounds, organic acids, or salts was monitored by absorbance measurements for 24 or 72 h. Three different ways of analyzing optical density (OD) curves were tested for the determination of MICs. Both quantitative methods gave similar MICs for most of the compounds. The semiquantitative method does not allow estimating the MIC for all compounds. Noticeable differences were found between MICs obtained for 24- or 72-h experiments, whatever the method used. The proposed methods and models can be used for the estimation of MICs from OD data. MICs could be used for a quantitative approach to Salmonella Typhimurium growth.

Document Type: Research article

Affiliations: 1: AgroParisTech, UMR763 Bioadhésion et Hygiène des Aliments, 1 avenue des Olympiades, F-91300 Massy, France; INRA, UMR763 Bioadhésion et Hygiène des Aliments, 25 rue de la République, F-91300 Massy, France; AFSSA DERNS, Unité d'Evaluation des Risques Biologiques, 23 avenue du Général Leclerc F-94700 Maisons-Alfort, France 2: AgroParisTech, UMR763 Bioadhésion et Hygiène des Aliments, 1 avenue des Olympiades, F-91300 Massy, France; INRA, UMR763 Bioadhésion et Hygiène des Aliments, 25 rue de la République, F-91300 Massy, France 3: AgroParisTech, UMR763 Bioadhésion et Hygiène des Aliments, 1 avenue des Olympiades, F-91300 Massy, France; INRA, UMR763 Bioadhésion et Hygiène des Aliments, 25 rue de la République, F-91300 Massy, France;, Tel: (33) 1 69 53 64 72, Fax: (33) 1 69 20 02 30, Email: florence.dubois-brissonnet@jouy.inra.fr

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