Authors: Guillier, L.¹; Nazer, A.I.²; Dubois-Brissonnet, F.³

Source: Journal of Food Protection®, Volume 70, Number 10, October 2007 , pp. 2243-2250(8)

Publisher: International Association for Food Protection

Abstract:

The aim of this study was to determine the MICs of 14 antimicrobials for Salmonella Typhimurium with three methods and to check the influence of experiment duration on the estimation of MICs. The growth of Salmonella Typhimurium in a brain heart infusion medium containing various concentrations of natural aromatic compounds, organic acids, or salts was monitored by absorbance measurements for 24 or 72 h. Three different ways of analyzing optical density (OD) curves were tested for the determination of MICs. Both quantitative methods gave similar MICs for most of the compounds. The semiquantitative method does not allow estimating the MIC for all compounds. Noticeable differences were found between MICs obtained for 24- or 72-h experiments, whatever the method used. The proposed methods and models can be used for the estimation of MICs from OD data. MICs could be used for a quantitative approach to Salmonella Typhimurium growth.

Document Type: Research article

Affiliations: 1: AgroParisTech, UMR763 Bioadhésion et Hygiène des Aliments, 1 avenue des Olympiades, F-91300 Massy, France; INRA, UMR763 Bioadhésion et Hygiène des Aliments, 25 rue de la République, F-91300 Massy, France; AFSSA DERNS, Unité d'Evaluation des Risques Biologiques, 23 avenue du Général Leclerc F-94700 Maisons-Alfort, France 2: AgroParisTech, UMR763 Bioadhésion et Hygiène des Aliments, 1 avenue des Olympiades, F-91300 Massy, France; INRA, UMR763 Bioadhésion et Hygiène des Aliments, 25 rue de la République, F-91300 Massy, France 3: AgroParisTech, UMR763 Bioadhésion et Hygiène des Aliments, 1 avenue des Olympiades, F-91300 Massy, France; INRA, UMR763 Bioadhésion et Hygiène des Aliments, 25 rue de la République, F-91300 Massy, France;, Tel: (33) 1 69 53 64 72, Fax: (33) 1 69 20 02 30, Email: florence.dubois-brissonnet@jouy.inra.fr

Publication date: 2007-10-01

More about this publication?

• IAFP members must first sign in on the right to access full text articles of JFP
  
  First published in 1937, the Journal of Food Protection^®, is a refereed monthly publication. Each issue contains scientific research and authoritative review articles reporting on a variety of topics in food science pertaining to food safety and quality. The Journal is internationally recognized as the leading publication in the field of food microbiology with a readership exceeding 11,000 scientists from 70 countries. The Journal of Food Protection^® is indexed in Index Medicus, Current Contents, BIOSIS, PubMed, Medline, and many others.
  
  Print and online subscriptions are available to Members and Institutional subscribers. Online visitors who are not IAFP Members or journal subscribers will be charged on a pay-per-view basis. Information can be obtained by calling +1 800.369.6337; +1 515.276.3344; fax: +1 515.276.8655, E-mail: info@foodprotection.org or Web site: www.foodprotection.org
• Information for Authors
• Submit a Paper
• Subscribe to this Title
• Membership Information
• Information for Advertisers
• ingentaconnect is not responsible for the content or availability of external websites

Related content

• In this: publication
• By this: publisher
• In this Subject: Nutrition & Food
• By this author: Guillier, L. ;  Nazer, A.I. ;  Dubois-Brissonnet, F.

Website © Publishing Technology. Article copyright remains with the publisher, society or author(s) as specified within the article.

• Terms and conditions
• Privacy policy
• Information for advertisers