IngentaConnect Media for the Aerobic Resuscitation of Campylobacter jejuni

Authors: Tangwatcharin, P.¹; Chanthachum, S.¹; Khopaibool, P.²; Chambers, J.R.³; Griffiths, M.W.⁴

Source: Journal of Food Protection®, Volume 70, Number 5, May 2007 , pp. 1099-1109(11)

Publisher: International Association for Food Protection

Abstract:

The microaerophilic nature of Campylobacter jejuni has complicated its recovery from human and animal sources. In this study, enhancement of the growth and aerotolerance of C. jejuni ATCC 35921 in nutrient broth no. 2 (NB2) was investigated. The efficiency of recovery of C. jejuni in NB2 containing FBP (0.025% [each] ferrous sulfate, sodium metabisulfite, and sodium pyruvate), 5% laked horse blood, hemin, Oxyrase, or activated charcoal in an aerobic atmosphere was compared with that obtained under microaerophilic incubation. The shortest lag time (λ) for cells grown aerobically was observed with NB2 supplemented with FBP, 5% laked horse blood, 0.01 g/liter of hemin, or 0.15 U/ml of Oxyrase. The efficacy of these media to resuscitate C. jejuni cells in late exponential phase, as well as cells subjected to stress induced by cold, heat, starvation, or acid, was determined in aerobic or microaerobic atmospheres. The λ of cells grown aerobically in NB2 containing both FBP and blood was similar to that obtained in the same medium incubated in a microaerobic environment (P > 0.05). However, the λ was longer during aerobic growth when low numbers of cells (approximately 1 log CFU/ml) in late exponential phase were used as the initial inoculum. The best recovery of stressed C. jejuni was observed in NB2 supplemented with FBP and blood and incubated aerobically. Enrichment in media incorporating FBP and 5% laked horse blood is a simple, convenient, and time-saving method to replace microaerophilic incubation methods for the resuscitation of C. jejuni.

Document Type: Research article

Affiliations: 1: Department of Food Science and Technology, Prince of Songkla University, Songkhla, 90112, Thailand 2: Department of Food Science and Technology, King Mongkut's Institute of Ladkrabang, Bangkok, 10520, Thailand 3: Food Research Program, Agriculture and Agri-Food Canada, Ontario, Canada N1G 5C9 4: Department of Food Science, University of Guelph, Guelph, Ontario, Canada N1G 2W1; Canadian Research Institute for Food Safety, University of Guelph, Guelph, Ontario, Canada N1G 2W1

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