Inactivation of Vibrio parahaemolyticus and Vibrio vulnificus in Phosphate-Buffered Saline and in Inoculated Whole Oysters by High-Pressure Processing
Abstract:Inactivation studies for Vibrio parahaemolyticus TX-2103 (serotype O3:K6) and Vibrio vulnificus MO-624 (clinical isolate) were conducted in phosphate-buffered saline (PBS) and in inoculated oysters under high-pressure processing conditions. V. parahaemolyticus was more resistant than V. vulnificus in PBS at all pressures and times. A 6-log reduction of V. parahaemolyticus and V. vulnificus in PBS at 241 MPa required 11 and 5 min, respectively, which included a 3-min pressure come-up time. A 4.5-log reduction of V. parahaemolyticus in oysters at 345 MPa required 7.7 min, which included a 6.7-min pressure come-up time. More than a 5.4-log reduction of V. vulnificus in oysters at 345 MPa occurred during the 6-min pressure come-up time. Both V. parahaemolyticus and V. vulnificus in PBS and in oysters were reduced to nondetectable numbers at 586 MPa during the 8- and 7-min pressure come-up times, respectively.
Document Type: Research Article
Affiliations: 1: Virginia Seafood Agricultural Research and Extension Center, Virginia Polytechnic Institute and State University, Virginia Sea Grant Program, 102 South King Street, Hampton, Virginia 23693, USA 2: Department of Microbiology, Oregon State University, Hatfield Marine Science Center, 2030 S.E. Marine Science Drive, Newport, Oregon 97365, USA 3: Department of Biological Systems Engineering, Virginia Polytechnic Institute and State University, 200 Seitz Hall, Blacksburg, Virginia 24060, USA
Publication date: March 1, 2006
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