Genetic Mechanisms Contributing to Reduced Tetracycline Susceptibility of Campylobacter Isolated from Organic and Conventional Dairy Farms in the Midwestern and Northeastern United States
Abstract:Campylobacter is one of the most common causes of gastroenteritis and can be acquired through contact with farm animals or the consumption of raw milk. Because of concerns over the role of food-producing animals in the dissemination of antimicrobial resistance to humans, we evaluated the prevalence of antimicrobial resistance in Campylobacter isolates from dairy farms and the genetic mechanism conferring the observed resistance. Evaluation of antimicrobial resistance was completed on 912 isolates from conventional and 304 isolates from organic dairy farms to eight drugs (azithromycin, chloramphenicol, ciprofloxacin, clindamycin, erythromycin, gentamicin, nalidixic acid, and tetracycline) with microbroth dilution. Resistance to seven of eight drugs was very low and did not differ by farm type. However, tetracycline resistance was common in Campylobacter isolated from both organic and conventional dairy farms, with 48 and 58% of isolates affected, respectively. By multiplex PCR, we determined that tetracycline resistance was highly associated with the carriage of tetO in Campylobacter isolates (χ2 = 124, P < 0.01, kappa = 0.86).
Document Type: Research Article
Affiliations: 1: Population Medicine Center, Michigan State University, East Lansing, Michigan 48824, USA; U.S. Drug Safety, P?zer Animal Health, Exton, PA 19520, USA 2: Population Medicine Center, Michigan State University, East Lansing, Michigan 48824, USA 3: College of Veterinary Medicine, Michigan State University, East Lansing, Michigan 48824, USA 4: College of Agriculture and Life Sciences, University of Wisconsin, Madison, Wisconsin 53706, USA 5: College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, USA 6: College of Veterinary Medicine, University of Minnesota, St. Paul, Minnesota 55108, USA 7: College of Veterinary Medicine, University of Minnesota, St. Paul, Minnesota 55108, USA; Centers for Epidemiology and Animal Health, USDA-APHIS-VS, Fort Collins, CO 80562, USA
Publication date: March 1, 2006
- IAFP Members with personal subscriptions to JFP Online: To access full-text JFP or JMFT articles, you must sign-in in the upper-right corner using your Ingenta sign-in details (your IAFP Member Login does not apply to this website). The Journal of Food Protection (JFP) is a refereed monthly publication. Each issue contains scientific research and authoritative review articles reporting on a variety of topics in food science pertaining to food safety and quality. The Journal is internationally recognized as the leading publication in the field of food microbiology with a readership exceeding 11,000 scientists from 70 countries. The Journal of Food Protection is indexed in Index Medicus, Current Contents, BIOSIS, PubMed, Medline, and many others.
Print and online subscriptions are available to IAFP Members and institutional subscribers. IAFP Members with a subscription to JFP Online will have access to all available JFP and JMFT content. Online visitors who are not IAFP Members or journal subscribers will be charged on a pay-per-view basis. Membership and subscription information is available at www.foodprotection.org.
- Information for Authors
- Submit a Paper
- Subscribe to this Title
- Membership Information
- Information for Advertisers
- ingentaconnect is not responsible for the content or availability of external websites