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Comparison of Methods for Recovering Vibrio cholerae O1 from Ice

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Abstract:

Alkaline peptone water (1% peptone, 1% NaCl, pH 8.5) and Trypticase soy yeast extract broth (TSYB) supplemented with 2.5% NaCl (pH 8.5) or 1% NaCl (pH 7.5) were evaluated as enrichment broths for the isolation of Vibrio cholerae O1 from ice. Thirty samples of sterile and nonsterile mineral water were inoculated with cell suspensions of this bacterium, quickly frozen, and stored for 3 days at −18°C. After thawing, samples were analyzed by a three-tube most-probable-number technique. Incubation in TSYB with 2.5% NaCl (pH 8.5) for 18 h at 37°C yielded the highest recovery of V. cholerae O1 cells (P < 0.05), a result that might be attributable to the nutrients and to the NaCl concentration of the TSYB, both of which would promote V. cholerae O1 growth and prevent the growth of competitive microbiota.

Document Type: Short Communication

Affiliations: 1: Departamento de Procesos Biológicos y Bioquímicos, Sección de Control de Calidad y Desarrollo de Nuevos Productos, Edificio de Química y Procesos, Universidad Simón Bolívar, Valle de Sartenejas, Caracas, Venezuela, Apdo Postal. No. 89000; Departamento de Tecnología de Alimentos, Nutrición y Bromatología, Facultad de Veterinaria, Universidad de Murcia, Campus de Espinardo, Apdo. 4021, 30071 Murcia, Spain 2: Departamento de Procesos Biológicos y Bioquímicos, Sección de Control de Calidad y Desarrollo de Nuevos Productos, Edificio de Química y Procesos, Universidad Simón Bolívar, Valle de Sartenejas, Caracas, Venezuela, Apdo Postal. No. 89000 3: Departamento de Tecnología de Alimentos, Nutrición y Bromatología, Facultad de Veterinaria, Universidad de Murcia, Campus de Espinardo, Apdo. 4021, 30071 Murcia, Spain

Publication date: January 1, 2006

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