Multicenter Validation of a Multiplex PCR Assay for Differentiating the Major Listeria monocytogenes Serovars 1/2a, 1/2b, 1/2c, and 4b: Toward an International Standard
Authors: Doumith, Michel1; Jacquet, Christine1; Gerner-Smidt, Peter2; Graves, Lewis M.3; Loncarevic, Semir4; Mathisen, Tone4; Morvan, Anne1; Salcedo, Celia5; Torpdahl, Mia2; Vazquez, Julio A.5; Martin, Paul1
Source: Journal of Food Protection®, Number 12, December 2005, pp. 2502-2720 , pp. 2648-2650(3)
Abstract:The performance of a multiplex PCR assay that separates the four major serovars of the pathogenic Listeria monocytogenes into four distinct PCR groups was evaluated through a multicenter typing study. Identical panels of 90 Listeria isolates were distributed to five participating laboratories that were blind to the nature of the isolates. Isolates were characterized using the previously standardized protocol. Overall concordance was 96.6 to 100%, sufficient for the assay to be used as an alternative to serotyping and confidently applied in laboratories involved in L. monocytogenes typing.
Document Type: Research Article
Affiliations: 1: Laboratoire des Listeria, Centre National de Référence des Listeria, World Health Organisation Collaborating Center for Foodborne Listeriosis, Institut Pasteur, 25-28 rue du Docteur Roux, 75724 Paris Cedex 15, France 2: Statens Serum Institut, Department of Bacteriology, Mycology and Parasitology, Artillerivej 5, 2300 Copenhagen, Denmark 3: Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA 4: Section for Feed and Food Microbiology, National Veterinary Institute, P.O. Box 8156 Dep., 0033 Oslo, Norway 5: Reference Laboratory for Listeria, National Institute of Health, Carlos III, Majadahonda, Madrid, Spain
Publication date: 2005-12-01
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