Skip to main content

Multiplex-Touchdown PCR Assay for the Detection and Genotyping of Helicobacter pylori from Artificially Contaminated Sheep Milk

The full text article is temporarily unavailable.

We apologise for the inconvenience. Please try again later.


Helicobacter pylori (Hp) is an organism commonly present worldwide in the human population, sometimes causing serious illnesses such as duodenal and gastric ulcers, adenocarcinoma of the stomach, and low-grade B-cell mucosa-associated lymphoid tissue lymphoma of the stomach. This article describes a multiplex-touchdown PCR method for the identification and genotyping (vacA—s1/m1, s1/m2, and s2/m2—and cagA genes) of Hp directly from sheep milk artificially contaminated with Hp strains from human gastric biopsies and with Hp ATCC 43504. The strains from humans carried s1/m2 cagA + and s2/m2 cagA allelic combinations, while the ATCC strains carried an s1/m1 cagA + allelic combination. The technique showed a sensitivity of 15 CFU/ml for species identification and of 1,500 CFU/ml for the detection of genes encoding for VacA and CagA. It has proven to be specific and rapid, and the authors suggest that it be used as a rapid screening method to ensure that sheep milk is uncontaminated with this organism.

Document Type: Research Article

Affiliations: 1: Department of Health and Animal Welfare–Faculty of Veterinary Medicine, Str. Prov. per Casamassima Km 3, 70010 Valenzano (Ba), Italy 2: Istituto Zooprofilattico Sperimentale della Puglia e Basilicata, V. Chiancolla n. 1, Putignano (Bari), Italy 3: Istituto Zooprofilattico Sperimentale della Sardegna, V. F. lli Kennedy, 08100, Nuoro, Italy

Publication date:

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more