Skip to main content

Acquisition of Bile Salt Resistance Promotes Antibiotic Susceptibility Changes in Bifidobacterium

Buy Article:

$37.00 plus tax (Refund Policy)

Abstract:

The effect of acquired resistance to bile on the antimicrobial sensitivity of two Bifidobacterium strains (Bifidobacterium animalis IPLA4549 and Bifidobacterium longum NIZO B667) was studied. The MICs of 23 different antibiotics belonging to the most clinically important groups were determined by using the Etest method, which comprises nonporous plastic strips calibrated with a predefined gradient of antibiotic concentrations covering 15 twofold dilutions. The strains were sensitive to most antibiotics assayed, although they tolerated relatively high concentrations of gentamicin, kanamycin, streptomycin, poly-myxin B, and ciprofloxacin (from 32 to more than 1,024 μg/ml). One of the bile-adapted strains was more strongly resistant to ceftazidime than was its parent bile-sensitive strain, and the other bile-adapted strain had increased resistance to tetracyclines. Therefore, to test the possibility that the acquisition of stable resistance to bile could be associated with a general increase in resistance to some antibiotics, we analyzed the sensitivities of four additional pairs of parent strains and their bile-adapted derivatives to ceftazidime and three tetracyclines (doxycycline, minocycline, and tetracycline). Three of the bile-resistant derivatives had increased resistance to ceftazidime (more than 256-fold) compared with their parents, and two had enhanced resistance to tetracyclines (at least 12-fold). Thus, the acquisition of bile salts resistance in Bifidobacterium induced modifications of the antibiotic resistance patterns. These results suggest that adaptation of probiotics to bile could also change their potential impact on intestinal microbiota, and this possibility deserves further attention.

Document Type: Short Communication

Affiliations: Instituto de Productos Lácteos de Asturias, Consejo Superior de Investigaciones Científicas, Ctra. Infiesto s/n, 33300 Villaviciosa, Asturias, Spain

Publication date: September 1, 2005

More about this publication?
  • IAFP Members with personal subscriptions to JFP Online: To access full-text JFP or JMFT articles, you must sign-in in the upper-right corner using your Ingenta sign-in details (your IAFP Member Login does not apply to this website).

    The Journal of Food Protection (JFP) is a refereed monthly publication. Each issue contains scientific research and authoritative review articles reporting on a variety of topics in food science pertaining to food safety and quality. The Journal is internationally recognized as the leading publication in the field of food microbiology with a readership exceeding 11,000 scientists from 70 countries. The Journal of Food Protection is indexed in Index Medicus, Current Contents, BIOSIS, PubMed, Medline, and many others.

    Print and online subscriptions are available to IAFP Members and institutional subscribers. IAFP Members with a subscription to JFP Online will have access to all available JFP and JMFT content. Online visitors who are not IAFP Members or journal subscribers will be charged on a pay-per-view basis. Membership and subscription information is available at www.foodprotection.org.
  • Information for Authors
  • Submit a Paper
  • Subscribe to this Title
  • Membership Information
  • Information for Advertisers
  • ingentaconnect is not responsible for the content or availability of external websites
iafp/jfp/2005/00000068/00000009/art00020
dcterms_title,dcterms_description,pub_keyword
6
5
20
40
5

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more