Multiplex PCR Assay Simplifies Serotyping and Sequence Typing of Listeria monocytogenes Associated with Human Outbreaks

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Listeria monocytogenes serotypes 1/2a and 4b are responsible for the majority of cases of human listeriosis worldwide. In this study, a multiplex PCR assay was developed to allow rapid identification and easily interpretable differentiation of serotypes 1/2a and 4b from other serotypes of L. monocytogenes by simultaneously targeting two virulence genes (inlB and inlC) and two serotype-specific genes (ORF2372 and lmo0171). A subsequent gel extraction and sequence typing analysis of the highly polymorphic intragenic regions in inlB and inlC simplified a previously developed multi–virulence-locus sequence typing scheme and provided discriminatory power for subtyping L. monocytogenes similar to pulsed-field gel electrophoresis analysis.

Document Type: Short Communication

Affiliations: Department of Food Science, Pennsylvania State University, University Park, Pennsylvania 16802-2504, USA

Publication date: September 1, 2005

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