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Evaluation of a Capillary Immunoassay System for Detection of Salmonella Typhimurium in Poultry Products

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Abstract:

A capillary immunoassay system was constructed and optimized for detection of Salmonella Typhimurium. The system consisted of a capillary bioseparator-bioreactor and a flow-injection electrochemical detector. Three methods were compared for immobilizing antibodies on the inner surface of silica capillary columns; these methods were based on the use of a homobifunctional cross-linker glutaraldehyde, a heterobifunctional cross-linker N-succinimidyl-4-maleimidobutyrate, and biotin-streptavidin chemistry, respectively. The glutaraldehyde method gave the best reproducibility with a relative standard deviation of 1 to 6% for detection of Salmonella Typhimurium. The optimized immunoassay system could detect Salmonella Typhimurium in chicken breast and ground turkey meats with a detection limit of 2.4 × 103 and 2.4 × 104 CFU/ml, respectively. The total detection time was less than 2.5 h without any preenrichment. When stored at 4°C, the immunocolumns could retain their activities for at least 3 months.

Document Type: Research Article

Affiliations: 1: Department of Food Science, University of Arkansas, Fayetteville, Arkansas 72701, USA 2: Department of Biological and Agricultural Engineering, University of Arkansas, Fayetteville, Arkansas 72701, USA 3: Department of Biological and Agricultural Engineering, University of Arkansas, Fayetteville, Arkansas 72701, USA; Center of Excellence for Poultry Science, University of Arkansas, Fayetteville, Arkansas 72701, USA

Publication date: September 1, 2005

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