Quantitative Detection of Species-Specific DNA in Feedstuffs and Fish Meals

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Abstract:

A sensitive and rapid method for the quantitative detection of bovine-, ovine-, swine-, and chicken-specific mitochondrial DNA sequences based on real-time PCR has been developed. The specificity of the primers and probes for real-time PCR has been tested using DNA samples of other vertebrate species that may also be present in rendered products. The quantitative detection was performed with dual-labeled probes (TaqMan) using absolute quantification with external standards of single species meat-and-bone meals. This method facilitates the detection of 0.01% of the target species–derived material in concentrate feed mixtures and fish meals.

Document Type: Research Article

Affiliations: Veterinary Research Institute, Hudcova 70, 621 32 Brno, Czech Republic

Publication date: June 1, 2005

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