Menadione-Catalyzed Luminol Chemiluminescence Assay for the Rapid Detection of Viable Bacteria in Foods under Aerobic Conditions

Authors: Kawasaki, S.1; Yamashoji, S.2; Asakawa, A.3; Isshiki, K.1; Kawamoto, Ands.1

Source: Journal of Food Protection®, Number 12, December 2004, pp. 2644-2857 , pp. 2767-2771(5)

Publisher: International Association for Food Protection

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A menadione-catalyzed luminol chemiluminescence assay was developed for the rapid detection and estimation of viable bacteria in foods. The principle of this assay is based on the extracellular menadione-catalyzed active oxygen spieces (O2- and H2O2 ) generated by the activity of NAD(P)H:menadione oxidoreductase in viable cells. This luminol chemiluminescence assay requires 10 min for the incubation of cells with menadione and then 2 s for the measurement of chemiluminescence intensity after an injection of luminol solution without the treatment of cell lysis. This method was evaluated using liquid food samples of milk, vegetable juice, green tea, and coffee spiked with Escherichia coli ATCC 25922. The study result revealed that E. coli contamination at 1 to 10 CFU/ml in these foods could be detected after incubation at 37°C for 7 h in an enrichment medium; however, the green tea and coffee samples requires filtration. This method could be a useful tool for the rapid evaluation of microbial food contamination.

Document Type: Research Article

Affiliations: 1: National Food Research Institute, Food Hygiene Research Team, 2-1-12 Kannondai, Tsukuba-shi, Ibaraki 305-8642, Japan 2: Nikken Biomedical Laboratory, 23 Teigaien, Oohashibe, Kumiyama-cho, Kuze-gun, Kyoto 613-0046, Japan 3: ATTO Corporation, 1-25-23 Hongou, Bunkyou-ku, Tokyo 113-8425, Japan

Publication date: December 1, 2004

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    First published in 1937, the Journal of Food Protection®, is a refereed monthly publication. Each issue contains scientific research and authoritative review articles reporting on a variety of topics in food science pertaining to food safety and quality. The Journal is internationally recognized as the leading publication in the field of food microbiology with a readership exceeding 11,000 scientists from 70 countries. The Journal of Food Protection® is indexed in Index Medicus, Current Contents, BIOSIS, PubMed, Medline, and many others.

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