Three intervention strategies—trisodium phosphate, lactic acid, and sodium metasilicate—were examined for their in vitro antimicrobial activities in water at room temperature against a three-strain cocktail of Escherichia coli O157:H7 and a three-strain cocktail of
''generic'' E. coli. Both initial inhibition and recovery of injured cells were monitored. When 3.0% (wt/wt) lactic acid, pH 2.4, was inoculated with E. coli O157:H7 (approximately 6 log CFU/ml), viable microorganisms were recovered after a 20-min exposure to the acid. After 20 min in
1.0% (wt/wt) trisodium phosphate, pH 12.0, no viable E. coli O157:H7 microorganisms were detected. Exposure of E. coli O157:H7 to sodium metasilicate (5 to 10 s) at concentrations as low as 0.6%, pH 12.1, resulted in 100% inhibition with no recoverable E. coli O157:H7. No
difference in inhibition profiles was detected between the E. coli O157:H7 and generic strains, suggesting that nonpathogenic strains may be used for in-plant sodium metasilicate studies.
Document Type: Research Article
Georgetown Technology Group, 11240 S. W. Lynnvale Drive, Portland, Oregon 97225 2:
NP Analytical Laboratories, Checkerboard Square, St. Louis, Missouri 63164 3:
Rhodia Foods, CN7500 Prospect Plains Road, Cranbury, New Jersey 08512, USA
Publication date: July 1, 2004
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