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Effect of Thermal Treatment during Processing of Orange Juice on the Enantiomeric Distribution of Chiral Terpenes

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Chiral terpenes in orange juices were examined by solid-phase microextraction-gas chromatography-mass spectrometry to study the dependence of their enantiomeric composition on the thermal treatment applied during the industrial manufacture. The experimental conditions used in the isolation and concentration of the compounds of interest produced relative standard deviations ranging from 2.9 to 15.1% when absolute areas were used and from 1.7 to 18.3% when normalized areas were used. Recovery varied between 8.8 and 56.1%, and detection limits ranged from 0.11 to 0.32 μg/ml. The enantiomeric compositions of the majority of the chiral terpenes varied within a reasonably narrow range. Nevertheless, the enantiomeric ratio of two monoterpene alcohols, α-terpineol and linalool, exhibited considerable variation according to the thermal treatment used in the manufacture of the juices. Therefore, the knowledge of the enantiomeric composition of α-terpineol and linalool might be useful for thermal treatment control purposes.


Document Type: Research Article

Affiliations: Instituto de Fermentaciones Industriales, CSIC, Juan de la Cierva 3, 28006 Madrid, Spain

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